Breast Tissue X-Ray
In our lab we XRay all breast bx and mastectomy specimens received in the
gross room. The procedure that we have used for several years is to ink the
specimen when it is received and let it sit overnight in formalin. The Path
Assistant will slice the breast, the next day, after it is oriented, in thin
slices and place on x ray film. After the breast has been x ray'd the long
pieces of tissue are layed flat on a tray covered in formal alcohol until
the resident looks at the film and identifies calcifications. The resident
then cuts the sections from the strips of tissue and blocks it for
processing that night. I would like to know what others are doing for their
breast protocols.
Frank Tringale
Supervisor of Histology Services
Stanford University Medical Center
----- Original Message -----
From: "HistoNet Server"
To: "HistoNet Server"
Sent: Tuesday, April 30, 2002 8:49 PM
Subject: Daily Digest
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 03:15:24 -0500
> From: T.Hacker@har.mrc.ac.uk
> Subject: (Fwd) RE: amyloid control
>
>
> - ------- Forwarded Message Follows -------
> From: Self
> To: Terry.Marshall@rgh-tr.trent.nhs.uk
> Subject: RE: amyloid control
> Send reply to: T.Hacker@har.mrc.ac.uk
> Date sent: Tue, 30 Apr 2002 08:41:05 +0100
>
> For your information it is very easy to induce Amyloid in mice as
> well as developing as a spontaneous or secondary disease. We
> used this model many years ago when I worked in the NHS which
> proved invaluable in understanding the disease and developing
> staining techniques.Luckily I have had some useful replies from
> the USA and UK to supply some material from animal species.
> Terry.
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 06:30:49 -0500
> From: "Edwards, R.E."
> Subject: RE: Third class countries?
>
>
>
> I thought the term "Third world country" had been replaced by the
PC
> term "Developing country", and if the USA is a First world country and
> Argentina a Third world country who are the Second world
countries???just a
> thought.............
>
> Richard Edwards
>
> Leicester......U.K......
>
> - -----Original Message-----
> From: Jasper, Thomas [mailto:TJasper@smdc.org]
> Sent: 30 April 2002 01:09
> To: 'Monson, Frederick C.'
> Cc: 'histonet@pathology.swmed.edu'
> Subject: RE: Third class countries?
>
>
> Very well said, Fred. I happen to have worked with and met some people
from
> 3rd world countries and overall I would say the biggest surprise to most
of
> them is that we are NOT as portrayed by their governments. By the same
token
> all Americans do not necessarily agree with foreign policy decisions made
by
> our government. That is just an example of the beauty of democracy, we can
> disagree and freely do so.
> I believe Glen Dawson restated a maxim not long ago on this posting about
> Americans, "I may not agree with you but I will defend your right to
> disagree with me to the end!" That was the jist anyway(sorry if I wasn't
> exact Glen)but I digress. The 3rd world people I've been fortunate enough
to
> meet have all been extremely intelligent, English often being a second or
> third language and the reason they were in America was due to this
> intelligence. More than a sharing of scientific information with these
> people I was fortunate receive unique perspectives on world issues.
> The other big surprise to many of these people is that there are poor and
> uneducated people in this country, obviously not comparable to other
places,
> however the streets are not paved with gold and it is not all milk and
honey
> flowing everywhere. America is not perfect...far from it, but as Fred
> pointed out there are a lot of people in this country that give a damn
about
> the rest of the world that is not so fortunate.
> Here's one for the Brits out there too, I've always loved what Winston
> Churchill said about democracy, "Indeed it has been said that democracy is
> the worst form of government except all those other forms that have been
> tried from time to time." I salute people everywhere like Augustin and
pray
> for their health and prosperity. To work and live as they do takes great
> fortitude and dignity!
> P.S. to the Brits I love watching your government at work on C-span, it is
> totally enlightening and high quality entertainment!
> > -----Original Message-----
> > From: Monson, Frederick C. [SMTP:fmonson@wcupa.edu]
> > Sent: Monday, April 29, 2002 12:50 PM
> > To: '=?UNKNOWN?Q?Agust=EDn?= Venzano'
> > Cc: 'List-HistoPath'
> > Subject: RE: Third class countries?
> >
> > Dear Augustin,
> >
> > If I remember correctly, when I first learned Cell Biology, it was
> > from a "de Robertis", and when I first taught Cell biology, it was again
> > with a text by a "de Robertis". If we do things correctly, we are sad
to
> > see a neighbor in trouble and hope he survives. If we do things without
> > thinking, sometimes we don't use the best terms to express that concern.
> >
> > Most of us in the USA who are alive have not really experienced a
> > day of true want, much less a week or a year, so we tend to be somewhat
> > dispassionate about such things.
> >
> > The third world may exist, and it may include some of our neighbors,
> > but we do not take pleasure or comfort from that. The biggest surprise
I
> > had in my life was the outburst of passionate desire for liberty from
the
> > peoples of the USSR and China barely a decade ago. After 70 years, one
> > thought that such passion would have been eliminated. It is obvious
that
> > WE, in the USA, have evolved something, both politically and
economically
> > special, that most people in the world would like to emulate in some
> > manner
> > compatible with their culture and history. It is unfortunate that so
few
> > succeed.
> >
> > We of the USA have made many mistakes while trying to insure the our
> > life, liberty and happiness in a world that is 'shrinking' every day.
We
> > have power, because sooner or later, every person or group that comes to
> > join us gets the opportunity to contribute to and benefit from the
system
> > of
> > government that we have developed. It is apparent, after two hundred
> > years,
> > that one reason for our success is that our 'ruling aristocracy' rises
> > from
> > the people, not the military or the landed rich. Furthermore, we have
> > been
> > fortunate that our laws do not permit our military to be used against
our
> > own people, that in addition to a stabilizing constitution we have a
Bill
> > of
> > Rights that is brief and, for the most part, unassailable.
> >
> > Tell us how to get it right, and if we can, we will! Did you know
> > that when I was young, grain sent to Northern African states, like Egypt
> > and
> > Libya, had the statement "From the People of the United States" removed
> > and
> > replaced by something that diminished our contribution. Yet, we
continued
> > to send the grain. After we had supported the revolution in Cuba,
Castro
> > formed a dictatorship. We have not always been at fault. How many
> > nations
> > in trouble have come to us and said, "Help us to get it right"? Usually
> > they say, "Give us the money, and leave us alone!"
> >
> > Respectful Regards and a Continuing Wish for Friendship,
> >
> > Fred Monson
> >
> > Frederick C. Monson, PhD
> > Center for Advanced Scientific Imaging
> > Schmucker II Science Center
> > West Chester University
> > South Church Street and Rosedale
> > West Chester, Pennsylvania, USA, 19383
> > Phone: 610-738-0437
> > FAX: 610-738-0437
> > fmonson@wcupa.edu
> > CASI URL: http://darwin.wcupa.edu/casi/
> > WCUPA URL: http://www.wcupa.edu/
> > Visitors URL: http://www.wcupa.edu/_visitors/
> >
> >
> >
> > > ----------
> > > From: =?UNKNOWN?Q?Agust=EDn?= Venzano
> > > Sent: Monday, April 29, 2002 8:16 AM
> > > To: HistoNet Server
> > > Subject: Third class countries?
> > >
> > > Dear Histonetters: My dear fatherland, the Argentine Republic, belongs
> > to
> > > the South American "Third World" a rather despective topic referred to
> > in
> > > a
> > > recent
> > > Digest. You are right, we belong to the Third World, but just as to
our
> > > awful economic status. Don't you ever make the mistake to judge a
> > foreign
> > > country on the basis of material aspects, leaving aside its human
> > being's
> > > spiritual value!
> > > Many Argentine cytizens are as valuable as First World's people and
they
> > > are
> > > certainly as clever as, or even more intelligent than them!
> > > We Argentine researchers form part of the Argentine Republic, so the
> > above
> > > mentioned principles should be applied to us as well. Do you know
what's
> > > the
> > > major difference between Argentine and American, European or Japanese
> > > researchers?
> > > Just one= The budget they have at their disposition, including their
> > > wages'
> > > level. Let's put forth an example :Our Institute, the National
> > Institute
> > > of
> > > Agricultural Research (INTA) is
> > > formed by top level specialists ( Please make a proper interpretation
of
> > > the
> > > term "Specialists" as this one is meant for both scientists and
> > technics)
> > > in
> > > Agronomic,
> > > Veterinary, Biological, etc. Sciences. Most of us have achieved a MSc
or
> > > PhD
> > > degree
> > > or attended training courses abroad. All of us do love our job, but
due
> > > to
> > > our spoiled economy
> > > the funds of all Argentine federal Institutions such as INTA have been
> > > severely restricted,
> > > so we often have to perform miracles to carry out our duties. The
> > current
> > > INTA's budget is hardly enough to pay salaries and basic operating
> > > services
> > > such as electric power, gas, telephone and Internet. There are no
funds
> > > for
> > > Investigation! All research groups get financial support through
> > > cooperation
> > > agreements with private companies or with fully developed foreign
> > > countries,
> > > or
> > > instead by performing charged analysis.
> > > I'm sure that my country's situation will improve in the future,
> > > otherwise all of us should commit suicide! But there are the love of
our
> > > wives and children, the love of people suffering hunger and misery,
the
> > > love
> > > for our jobs, the love for our fatherland and many other values, which
> > > help
> > > us keeping up with Argentina's economic disaster.
> > >
> > > Sincerely yours Histonetters
> > >
> > > Agustin Jose Venzano Halliburton
> > > DVM-Pathology Group
> > > INTA Castelar, Argentina
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
> > >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 06:45:47 -0500
> From: "Mcleod, H, Heather"
> Subject: FAS - Jeff Silverman
>
> Dear Histonetters
>
> I have asked before whether anyone has used an antibody to Fatty Acid
> Synthase. I unluckily had no response. However I have just been
> through a file of interesting histonet contributions that I keep. I
> noticed that Jeff Silverman of Peptolab offered information on the
> staining of adipocytes AND he mentioned FAS. Jeff, can you throw any
> light on the availability of this antibody for FFPE material? I have
> searched many websites but I have not been able to find one.
> hopeful and thankful
>
> Heather Mcleod
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 08:15:34 -0500
> From: E Sharon Shields
> Subject: DAB and hematoxylin
>
> Hello Fellow Histonetters,
> I have a question I hope you can answer since it has caused me lots of
grief.
> Why will IHC slides not signal with DAB if they where first stained with
> Mayers Hematoxylin before the DAB was dropped on? On several occasions,
this
> morning being the latest, Hematoxylin was mistakenly drop on the slides
before
> the DAB. Part of the controls had DAB dropped on them and the rest where
> destained with 70% acid alcohol before the DAB was dropped. None of the
> control worked. WHY??? These are controls that I know are staining
nicely.
> As I said this is not the first time this has happened. WE need help!!!!!
> Thanks,
> E.S. Shields
> Baptist Hospital of E TN
> Knoxville, TN
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 08:15:52 -0500
> From: Tim Webster
> Subject: RE: Breathers (was Microchatter chit chat)
>
> Normally a "breather"; but as I'm coming down with the 'flu, right now I'm
a
> "huffer" - which is just one step away from being a "gasper" - at which
> point I'm going home!
>
> Breath deep everyone!
>
> Tim
>
> Tim Webster RAMC
> Histology Specialist
> Northwestern Medical Center
> Fairfield Street
> St Albans, VT 05478
> (802) 524-1070
> Twebster@nmcinc.org
>
> - -----Original Message-----
> From: Noreen Gilman [mailto:Ngilman@nbhd.org]
> Sent: Monday, April 29, 2002 10:56 AM
> To: Allison@Cardiff.ac.uk; histonet@pathology.swmed.edu
> Subject: Re: Breathers (was Microchatter chit chat)
>
>
> No question about it, I'm a breather!
> Noreen
>
> Noreen Gilman, B.S., H.T.(ASCP) CLS
> Histopathology Supervisor
> Broward General Medical Center
> Ft. Lauderdale, FL 33316
> 954.355.5592 Phone
> 954.355.4139 Fax
> 954-387-0213 Pager
>
> >>> RUSS ALLISON 04/29/02 05:00AM >>>
> I'm on the side of the breathers.
> Will the results be age related?
>
> Russ Allison,
> Dental School
> Cardiff
> Wales
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 08:45:42 -0500
> From: Kappeler Andi
> Subject: Re: DAB and hematoxylin
>
> Hi Sharon
> Hematoxylin has a pH of approximately 2 to 2.5 ... and at this pH all your
> antibodies will readily dissociate from their respective antigens, meaning
> that all your staining efforts of the last couple of hours are just gone.
No
> way to save the job, just start over. Sorry to tell you this, but that's
the
> way it is. Low pH (hematoxylin, acid alcohol, whatever...) - no more bound
> antibodies ... and all these low pH reagents will only take seconds to
> destroy your previous work! Hope this helps.
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
>
> - -----Ursprungliche Nachricht-----
> Von: E Sharon Shields
> An:
> Gesendet: Dienstag, 30. April 2002 15:01
> Betreff: DAB and hematoxylin
>
>
> > Hello Fellow Histonetters,
> > I have a question I hope you can answer since it has caused me lots of
> grief.
> > Why will IHC slides not signal with DAB if they where first stained with
> Mayers Hematoxylin before the DAB was dropped on? On several occasions,
this
> morning being the latest, Hematoxylin was mistakenly drop on the slides
> before the DAB. Part of the controls had DAB dropped on them and the
rest
> where destained with 70% acid alcohol before the DAB was dropped. None of
> the control worked. WHY??? These are controls that I know are staining
> nicely. As I said this is not the first time this has happened. WE need
> help!!!!!
> > Thanks,
> > E.S. Shields
> > Baptist Hospital of E TN
> > Knoxville, TN
> >
> >
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 09:00:41 -0500
> From: HornHV@archildrens.org
> Subject: RE: Histotech Day?
>
> While I won't argue the point we are often treated as the "stepchildren"
of
> the laboratory, our laboratory recognizes us all on laboratory week. We
> had something special everyday of lab week. Contests, games, breakfasts,
> lunches, gifts and a note from management about their appreciation of
> everyone's hard work. It was a fun week for us all.
> Hazel
>
> > -----Original Message-----
> > From: Angel92764@aol.com [SMTP:Angel92764@aol.com]
> > Sent: Monday, April 29, 2002 8:55 PM
> > To: pmarcum@polysciences.com; histonet@pathology.swmed.edu
> > Subject: Re: Histotech Day?
> >
> > We are over looked and under appreciated. We deserve a day of
> > recognition.
> > How do we go about receiving that?
> >
> > Jeanie Wade, H. T. (ASCP)
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 09:00:59 -0500
> From: marjorie lehman
> Subject: OT( scientific, not silly) Teratology
>
> I'll bet someone on this list can help a colleague of mine.
> He is trying to find the definition of "lorata" used in connection with
> teratology studies. The context is that after XXXX was applied to the skin
of
> dam lorata appeared on pups at (whatever) age.
> We can't find it in dictionaries, texts or on the net.
> We'd really like to know what this is. Any help will be appreciated.
> Marge Lehman
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 09:15:55 -0500
> From: Linda Jenkins
> Subject: LInda Jenkins; Can you any anyone in Histo land help with this?
>
> Hi, Ian!
> You Wrote: "Linda, I understand you did a workshop for the
> National Society of
> Histotechnology: Callis G. Clark L, Jenkins L et al: "Let's do hard
tissue"
> National Society of Histotechnology Workshop No 3, Albuquerque,NM, 1996.
> Do you have this still available. Can you fax of e-mail me a copy.
> Or any one who has a copy?"
>
> I think I still have a copy of the handout. Seeing as how it contains
many
> pages, I will snail mail it to you. Maybe it's time to think about
> repeating this workshop...hmmm!
> Best wishes from a fellow "bonehead",
> Linda
>
> Linda Jenkins, HT
> Clemson University
> Dept. of Bioengineering
> Clemson, SC 29634-0905
> 864.656.5553
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 09:20:09 -0500
> From: Mark.Lewis@thermoshandon.com
> Subject: Bioloid Wax
>
> Does anyone know where I can obtain Bioloid wax?
>
> Can anyone tell me what the properties of this wax are ?
>
> I understand it is used primarily for processing & sectioning of eyes.
>
> Best regards,
>
> Mark
>
> Mark Lewis
> Product Specialist
> Thermo Shandon
> 171 Industry Drive, Pittsburgh, PA 15275 USA
> Direct: (412) 747-4013
> Fax: (412) 788-1097
> E-mail: mark.lewis@thermoshandon.com
>
> This e-mail message and all attachments transmitted herewith are trade
> secret and/or confidential information intended only for the viewing and
> use of the addressee. If the reader of this message is not the intended
> recipient, you are hereby notified that any review, use, communication,
> dissemination, distribution, or copying of this communication is
> prohibited. If you have received this communication in error, please
> notify the sender immediately by telephone, or electronic mail, and delete
> this message and all copies and backups thereof. Thank you for your
> cooperation.
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:00:40 -0500
> From: Linda Jenkins
> Subject: Jenkins decalcifier
>
> Sorry, Patsy...I can't lay claim to this formula...sounds interesting
though!
> Linda
>
> Linda Jenkins, HT
> Clemson University
> Dept. of Bioengineering
> Clemson, SC 29634-0905
> 864.656.5553
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:00:55 -0500
> From: Xorren@aol.com
> Subject: Job question
>
> Hello everyone,
> does anyone know of any histologist position or cytology prep positons in
New
> York
> or Virginia?
> Additionally, there will be an opening in NYC at the New York Eye and Ear
> Infirmary.
>
> Thanks for the info in advance !!
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:07:51 -0500
> From: Linda Jenkins
> Subject: NSH awards
>
> Procrastinators, Unite!
> May 1st is the deadline for all NSH awards. The nomination form
> is in the Spring 2001 "NSH in Action" newsletter. Being a charter member
> of the procrastinator's club, I just sent in my first nomination. There
> are some really great people out there in histoland. Bertie said as long
> as you fax the nomination form, the NSH office will accept the written
form
> through the mail.
> Linda
>
> Linda Jenkins, HT
> Clemson University
> Dept. of Bioengineering
> Clemson, SC 29634-0905
> 864.656.5553
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:08:11 -0500
> From: Jennifer MacDonald
> Subject: Re: Bone Marrow Smears - Perl's
>
> One minute in methyl alcohol.
>
>
>
> - ----- Original Message -----
> From: "Aidan Schurr"
> To:
> Sent: Monday, April 29, 2002 5:23 PM
> Subject: Bone Marrow Smears - Perl's
>
>
> > Gidday all.
> >
> > We do Perl's Prussian Blue stains on Bone Marrow smears for Haematology.
> Just wondering if anyone else out there does this also, and if so, what
(if
> anything) you fix the smears in before staining.
> >
> > Cheers,
> > Aidan
> >
> >
> > __
> >
> > aidan schurr b.m.l.sc
> > section head, histology
> > hutt valley district health board
> > lower hutt
> > new zealand
> >
> > aidan.schurr@hvh.co.nz
> > ++64 4 570 9173 (direct)
> > ++64 4 570 9214 (fax)
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:30:46 -0500
> From: nina leek
> Subject: Re: OT( scientific, not silly) Teratology
>
> Marjorie:
> My OED gives "lore - alt. lora" to mean a strap-like appendage in or on
> animals (among other meanings). If it is derived from Latin, and of the
same
> declension(?) as "stigma", the plural would be "lorata".
> My guess is that they are tissue flaps, perhaps like those weird things
goats
> have on their necks.
> The wonders of language!
> Adrian Leek.
>
>
>
> marjorie lehman wrote:
>
> > I'll bet someone on this list can help a colleague of mine.
> > He is trying to find the definition of "lorata" used in connection with
> > teratology studies. The context is that after XXXX was applied to the
skin
> of
> > dam lorata appeared on pups at (whatever) age.
> > We can't find it in dictionaries, texts or on the net.
> > We'd really like to know what this is. Any help will be appreciated.
> > Marge Lehman
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 10:31:21 -0500
> From: CMCCOLLOUGH@dnr.state.md.us
> Subject: RE: OT( scientific, not silly) Teratology
>
> Marjorie:
>
> Lorate is defined as strap-shaped. Perhaps lorata are strap-like
> 'somethings', markings, maybe?
>
> Regards -
> Carol
> *********************
> Carol B. McCollough, HT/HTL(ASCP)
> Diagnostics & Histology Laboratory Manager
> Maryland Department of Natural Resources
> Cooperative Oxford Laboratory
> 904 S. Morris Street
> Oxford, Maryland 21654
> cmccollough@dnr.state.md.us
>
>
> - -----Original Message-----
> From: marjorie lehman [mailto:Marjorie.Lehman@unilever.com]
> Sent: Tuesday, April 30, 2002 9:48 AM
> To: histonet
> Subject: OT( scientific, not silly) Teratology
>
>
> I'll bet someone on this list can help a colleague of mine.
> He is trying to find the definition of "lorata" used in connection with
> teratology studies. The context is that after XXXX was applied to the skin
> of
> dam lorata appeared on pups at (whatever) age.
> We can't find it in dictionaries, texts or on the net.
> We'd really like to know what this is. Any help will be appreciated.
> Marge Lehman
> ###########################################
>
> This message has been scanned for viruses.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 11:30:55 -0500
> From: "Histologyjobs.com"
> Subject: fyi
>
> Hello Histonetters,
>
> We have added Pathology Assistants as a new category.
>
> We have also added the following jobs:
> Histology Tech Supervisor-Baton Rouge, LA-Dynacare
> Histology Technician-Dallas, TX-Methodist Hospitals of Dallas
> Histotech Supervisor-Portsmouth, NH-Path Lab, Inc.
> Histology/Cytology Product Manager- Charlotte, NC-Premier, Inc.
> Just to name a few.
>
> Please go to http://www.histologyjobs.com to view
> all postings across the country or to post your
> open positions.
>
> Thanks,
> Scott Glasgow
> VP of Operations
> http://www.histologyjobs.com
> http://www.cytologyjobs.com
> http://www.jobs4medtechs.com
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 12:14:04 -0500
> From: RSRICHMOND@aol.com
> Subject: Re: OT( scientific, not silly) Teratology
>
> Marge Lehman asks:
>
> >>I'll bet someone on this list can help a colleague of mine. He is trying
to
> find the definition of "lorata" used in connection with teratology
studies.
> The context is that after XXXX was applied to the skin of dam lorata
appeared
> on pups at (whatever) age. We can't find it in dictionaries, texts or on
the
> net.<<
>
> I don't know this word, and a quick check of the Oxford English Dictionary
> and my father's 1920 Dorland's medical dictionary (both repositories of
rare
> and lost words) turns up nothing at all.
>
> The word teratology comes from the Greek 'teras' (plural 'terata') - the
> medical word teratoma comes from it, as does the Systeme Internationale
> (metric) prefix tera- (a thousand giga- or a thousandth of a peta-) -
meaning
> "monster".
>
> I suspect that the word "lorata" is a misreading of handwriting, possibly
for
> "terata" though I don't think that this word is ever used for ordinary
> tumors. ("*Terata appeared on pups." does not strike me as a likely
usage.)
>
> A Latin word lorum 'a strap or thong', plural lora, produces a number of
> obscure words in the OED. - A reference to a minor poem of Vergil
(Moretum,
> the Cheese and Garlic Dip) uses lorata for a "thong-encircled yoke" so
that
> conceivably the usage could refer to limb-reduction deformities produced
by
> congenital bands.
>
> A Google search is complicated by the fact that lorata is a common species
> name (such as Sterna lorata, the Peruvian tern).
>
> There - I believe I have left no stone unturned, and no tern unstoned, and
> (if indeed we're painting the butts of mama rats) no stern untoned.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 12:42:56 -0500
> From: "Diane G. Miller"
> Subject: AutoAligner
>
>
> Has anyone working in EM or Histology used a software program called
> AutoAligner? It's used by putting digital images of your sections into
the
> program, and then it helps you reconstruct it into a 3D image, and does
> measurements.
>
> If someone is using another program to do the same thing, would you please
> back channel me with the information.
>
>
> Thanks
> Diane
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_e+CNF2PqnfVKsWorY22Csw)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_e+CNF2PqnfVKsWorY22Csw)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Has anyone working in EM or Histology used a
> software program called AutoAligner? It's used by putting
digital
> images of your sections into the program, and then it helps
> you reconstruct
> it into a 3D image, and does measurements.
>
> If someone is using another program to do the
> same
> thing, would you please back channel me with the information.
>
>
> Thanks
> Diane
>
> - --Boundary_(ID_e+CNF2PqnfVKsWorY22Csw)--
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 13:29:41 -0500
> From: "DeLovino, Salvacion S."
> Subject: RE: Microchatter chit chat
>
> Breather here....
>
> > ----------
> > From: Bryan Hewlett[SMTP:bhewlett@cogeco.ca]
> > Sent: Sunday, April 28, 2002 11:34 AM
> > To: Jeff Silverman; histonet@pathology.swmed.edu
> > Subject: Re: Microchatter chit chat
> >
> > Jeff,
> >
> > In our past discussions about things histological, I suspected that you
> > were
> > a fellow 'Breather'!
> > Not a 'Heavy Breather' mind you, but a possessor and practitioner of
that
> > magic ingredient in the microtomy skill mix.
> > It would be intriguing to pole the 'netters', in order to find the ratio
> > of
> > 'Breathers' vs 'Non-Breathers', how about it folks?
> > It would also be intriguing to find out why the 'Breathers' think it
> > works?
> > We're not talking 'Puffing' here folks, that's the skill used mainly by
> > 'Blowers' to prevent section roll-up and/or to clear the knife. We're
> > talking 'Huffing', that arcane, gentle, open mouthed art that Jeff
> > describes.
> >
> > Bryan
> >
> > Bryan R. Hewlett
> > Technical Specialist
> > Anatomical Pathology
> > Hamilton Regional Laboratory Medicine Program
> > Ontario, Canada
> >
> >
> >
> > ----- Original Message -----
> > From: "Jeff Silverman"
> > To:
> > Sent: Sunday, April 28, 2002 12:49 PM
> > Subject: Microchatter chit chat
> >
> >
> > > Microchatter got you down? Face, chill on ice, section the block
slowly
> > and
> > > evenly while breathing hot breath on the block face (like trying to
fog
> > a
> > > mirror). Also, use plain old Paraplast. I have enough to do without
> > worrying
> > > whether I am mistreating or offending the mystery polymers and
> > plasticizers
> > > in some newfangled embedding medium. When I switched back to Paraplast
> > from
> > > one of those s0-called improved mixtures, all microtomy problems in my
> > lab
> > > disappeared. Just my opinion and experience.
> > > Jeff Silverman HT HTL QIHC (ASCP)
> > > Southside Hospital
> > > Bay Shore NY USA
> > >
> > >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 13:30:27 -0500
> From: Colleen Forster
> Subject: ???? job in NYC
>
> To the person looking for a job in NYC, read the following. Be sure to
> make note that the salary
> could be adjusted based on education and experience. I have worked in
> this lab for 5+ years and have
> enjoyed it very much. It will be moving to Cornell in Manhattan in June.
> If you have any other
> questions please call on Dr. Ross at the numbers listed below.
>
> Colleen Forster
> U of MN, Dept. of MN
> 612-626-2477
>
>
> A position is available at the Medical College of Cornell University for
> a full
> time research histology technician starting July 1, 2002. The laboratory
> works
> on molecular genetic problems in the formation of brain. Multiple mouse
> models
> are used in the study of human disorders of brain development.
>
> Questions that are being addressed fall into areas that range from cell
> cycle
> regulation to cell signaling and adhesion to motility and modulation of
> the
> neuronal cytoskeleton, working with genetic mouse models of human
> disease. These
> questions have relevance not only for human development but also for the
> study
> of epilepsy and behavioral disorders.
>
> While a background in neurobiology is desirable, it is not necessary.
> Skills are
> sought in the fixation and blocking of tissue, production of tissue
> sections
> from paraffin embedded specimens, cryostat sectioning and vibratome
> sectioning.
> Standard histological stains such as hematoxylin and eosin will be used.
>
> Experience in immunohistochemical labeling of antigens in tissue
> sections using
> HRP and Fluorescence detection methods is essential. The laboratory
> relies
> heavily on the use of a semi-automated tissue staining apparatus for
> high
> throughput processing of slides and appropriate training can be
> provided. Manual
> processing will also be used for special applications.
>
> Candidates should have a BA degree or equivalent and be a graduate of an
>
> established histology technician training program with at least 2 years
> of
> postgraduate experience in either a medical diagnostic or research
> laboratory.
> More postgraduate experience is desirable. Salary level begins at
> $28,000 and
> can be adjusted according to the candidateOs level of experience.
>
> Those interested should speak with Dr. Ross to explore a career in
> histology
> research in the area of neurogenetics and brain development. Contact Dr.
> Ross
> through Ravi Singh, at 212-746-5550 or directly at 612-626-2499 or
> 612-207-7393.
> She can also be reached by e-mail at rossx001@umn.edu.
>
>
> M. Elizabeth Ross, M.D., Ph.D.
> Professor,
> Laboratory of Developmental Neurogenetics
> Weill Medical College of Cornell University
> 1300 York Ave.
> New York, NY 10021
>
>
> __________________________________________________
> M. Elizabeth Ross, MD, PhD
> Head, Laboratory of Neurogenetics
> and Development
> Department of Neurology and Neuroscience Shipping Address:
> Weill Medical College of Cornell University Department of
> Neurology
> 525 East 68th Street, F610 Diehl Hall, Room 698
>
> New York, NY 10021 505 Essex Street SE
> Phone 612-626-2499; Fax 612-626-2639 Minneapolis, MN
> 55455
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 15:02:58 -0500
> From: Steve.Scholz@osfhealthcare.org
> Subject: School in Deep Freeze
>
> Histo Net:
>
> What was the name of that school in Minneapolis, MN that has a Histology
> program? How can I get in contact with them?
>
> I know somebody that my be interested in going this route to become a
Histo
> Tech. The school I went to in Eau Claire, WI has since shut down their
> program.
>
> Thanks in advance.
>
> Stephen J. Scholz HT(ASCP)
> Histology Laboratory
> OSF St. Anthony Medical Center
> Rockford Illinois
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 15:03:59 -0500
> From: "Charles W. Scouten, Ph.D."
> Subject: RE: A question to the Histonet Webmaster
>
> Histonet.org is only for pictures. "The histonet" may be subscribed too
> by sending an email to histonet@pathology.swmed.edu with the word
> Subscribe in the subject line, and nothing at all in the message area.
> You will start getting email from them. After subscribing, you may post
> messages. You may also unsubscribe the same way. To post a message,
> just send a message in "Plain Text" format to
> histonet@pathology.swmed.edu.
>
> Cordially,
>
> Charles W. Scouten, Ph.D.
> myNeuroLab.com
> 5918 Evergreen Blvd.
> St. Louis, MO 63134
> Ph: 314 522 0300
> FAX 314 522 0277
> cwscouten@myneurolab.com
> www.myneurolab.com
>
>
> - -----Original Message-----
> From: webmaster
> Sent: Tuesday, April 30, 2002 1:00 PM
> To: webmaster
> Subject: A question to the Histonet Webmaster
>
>
> textfield = spotmed@aol.com
>
> textfield2 = Cindy
>
> textfield3 = How do you post a message to histonet? I can't find anyway
> clearly marked.
>
> Submit = Email
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 15:04:16 -0500
> From: "Sennello, Gina"
> Subject: RE: OT( scientific, not silly) Teratology
>
> I asked someone who does teratology studies and he agrees that the word
is
> probably terata. In that context it means that the pups were mal-formed
in
> some way.
> Gina Sennello
> OSIP
> Boulder, CO
>
>
>
> - -----Original Message-----
> From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com]
> Sent: Tuesday, April 30, 2002 11:05 AM
> To: histonet@pathology.swmed.edu
> Subject: Re: OT( scientific, not silly) Teratology
>
>
> Marge Lehman asks:
>
> >>I'll bet someone on this list can help a colleague of mine. He is trying
> to
> find the definition of "lorata" used in connection with teratology
studies.
> The context is that after XXXX was applied to the skin of dam lorata
> appeared
> on pups at (whatever) age. We can't find it in dictionaries, texts or on
the
>
> net.<<
>
> I don't know this word, and a quick check of the Oxford English Dictionary
> and my father's 1920 Dorland's medical dictionary (both repositories of
rare
>
> and lost words) turns up nothing at all.
>
> The word teratology comes from the Greek 'teras' (plural 'terata') - the
> medical word teratoma comes from it, as does the Systeme Internationale
> (metric) prefix tera- (a thousand giga- or a thousandth of a peta-) -
> meaning
> "monster".
>
> I suspect that the word "lorata" is a misreading of handwriting, possibly
> for
> "terata" though I don't think that this word is ever used for ordinary
> tumors. ("*Terata appeared on pups." does not strike me as a likely
usage.)
>
> A Latin word lorum 'a strap or thong', plural lora, produces a number of
> obscure words in the OED. - A reference to a minor poem of Vergil
(Moretum,
> the Cheese and Garlic Dip) uses lorata for a "thong-encircled yoke" so
that
>
> conceivably the usage could refer to limb-reduction deformities produced
by
> congenital bands.
>
> A Google search is complicated by the fact that lorata is a common species
> name (such as Sterna lorata, the Peruvian tern).
>
> There - I believe I have left no stone unturned, and no tern unstoned, and
> (if indeed we're painting the butts of mama rats) no stern untoned.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 16:45:47 -0500
> From: Amos Brooks
> Subject: Re: DAB and hematoxylin
>
> Hi Andi,
> Just a quick dumb question 'bout that ... Wouldn't the Mayer's
> hematoxylin disassociate the Ab/Ag complex after the DAB was added too? I
am
> probably overlooking something and thereby asking a dumb question but the
> disassociation of the antibody should occur with or without the DAB when
in
> the hematoxylin if that was the cause. I don't have any other answer
except
> to try another hematoxylin and see if it still happens, or to be REALLY
> careful to counterstain only after the DAB is on (way to state the obvious
> right).
> Amos Brooks
>
> - ----- Original Message -----
> From: "Kappeler Andi"
> To: "Histonet" ; "E Sharon Shields"
>
> Sent: Tuesday, April 30, 2002 9:42 AM
> Subject: Re: DAB and hematoxylin
>
>
> Hi Sharon
> Hematoxylin has a pH of approximately 2 to 2.5 ... and at this pH all your
> antibodies will readily dissociate from their respective antigens, meaning
> that all your staining efforts of the last couple of hours are just gone.
No
> way to save the job, just start over. Sorry to tell you this, but that's
the
> way it is. Low pH (hematoxylin, acid alcohol, whatever...) - no more bound
> antibodies ... and all these low pH reagents will only take seconds to
> destroy your previous work! Hope this helps.
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
>
> - -----Ursprungliche Nachricht-----
> Von: E Sharon Shields
> An:
> Gesendet: Dienstag, 30. April 2002 15:01
> Betreff: DAB and hematoxylin
>
>
> > Hello Fellow Histonetters,
> > I have a question I hope you can answer since it has caused me lots of
> grief.
> > Why will IHC slides not signal with DAB if they where first stained with
> Mayers Hematoxylin before the DAB was dropped on? On several occasions,
this
> morning being the latest, Hematoxylin was mistakenly drop on the slides
> before the DAB. Part of the controls had DAB dropped on them and the
rest
> where destained with 70% acid alcohol before the DAB was dropped. None of
> the control worked. WHY??? These are controls that I know are staining
> nicely. As I said this is not the first time this has happened. WE need
> help!!!!!
> > Thanks,
> > E.S. Shields
> > Baptist Hospital of E TN
> > Knoxville, TN
> >
> >
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 16:46:16 -0500
> From: Tara Miller
> Subject: Re: GI biopsies - watch the heat
>
> I concur with Frank. Don't let the tissue get to hard and dry in the first
> place. We haven't needed to decrease our alcohol time (as our GI biopsies
> run with the rest of our tissues), but we remove our biopsies from the
> processor before the final paraffin. We simply put the biopsies (including
> biopsies that aren't GIs)in a separate basket for easy removal, and wait
for
> the retort to hit "ambient" so we can sneak them out. They're fully
> infiltrated but not "cooked". This works especially well with needle
> biopsies for liver and prostate.
>
> Tara Oakes,H.T.
> Central DuPage Hospital
>
>
> >From: FRANK TRINGALE
> >To: HistoNet Server
> >Subject: GI Biopsies
> >Date: Sun, 28 Apr 2002 16:58:36 -0700
> >
> >We were having the same problem with chatter on GI Biopsies. We tried
> >various ways of soaking the blocks while sectioning but this did not
solve
> >our problem. We have dedicated a VIP processor for GI BXs with a much
> >shorter cycle. The longer times in alcohol and warm paraffin tend to dry
> >out these specimens causing the chatter upon sectioning.
> >Frank Tringale
> >Supervisor Histology Services
> >Stanford Medical Center
>
>
> _________________________________________________________________
> Send and receive Hotmail on your mobile device: http://mobile.msn.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 16:46:41 -0500
> From: Maria Mejia
> Subject: research contract histo work
>
> Our research laboratory is in the working process of accepting outside
> histology
> contract work. I would like to know what others are "charging" for this
> type of
> service.
>
> We are mainly interested in knowing what to charge for receiveing fixed
> cortical
> brain tissues that need to be sectioned thick, on the sliding microtome,
> mounted and
> then stained with Nissl stain. Please contact me directly. Any advice or
> information
> on this matter will be most appreciated.
>
> regards
> Maria Mejia
> Smith-Kettlewell Eye Res. Inst.
> S.F.CA.
> email address: maria@ski.org
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 16:47:16 -0500
> From: Melissa Jensen
> Subject: Non Toluene
>
>
> Looking for a non Toluene based mounting media,that will work with xylene
> substitutes....Anyone using this? Thanks.
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_8gZHKjyEGfd9pzmi5s8Z4w)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_8gZHKjyEGfd9pzmi5s8Z4w)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Looking for a non Toluene based mounting
> media,that
> will work with xylene substitutes....Anyone using this?
> Thanks.
>
> - --Boundary_(ID_8gZHKjyEGfd9pzmi5s8Z4w)--
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 17:02:58 -0500
> From: SPOTMED@aol.com
> Subject: Used Equipment
>
> Our lab is currently looking to replace a Leitz 1510 microtome and obtain
a
> used embedding center (as a back up for extremely busy days).
> Does anyone have any used newer model microtome or embedding centers, or
do
> you know of anyone who does?
>
> Cindy DuBois
> Delta Pathology Associates
> Stockton, CA
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 17:03:49 -0500
> From: SPOTMED@aol.com
> Subject: Used Equipment
>
> Our lab is currently looking to replace a Leitz 1510 microtome and obtain
a
> used embedding center (as a back up for extremely busy days).
> Does anyone have any used newer model microtome or embedding centers, or
do
> you know of anyone who does?
>
> Cindy DuBois
> Delta Pathology Associates
> Stockton, CA
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 17:04:10 -0500
> From: TJasper@smdc.org
> Subject: RE: School in Deep Freeze
>
> Steve,
> The school is now called Argosy University(formerly MIM). Bob Brunner runs
> the program his phone # is (952)252-0290. Good luck!
>
> > -----Original Message-----
> > From: Steve.Scholz@osfhealthcare.org
[SMTP:Steve.Scholz@osfhealthcare.org]
> > Sent: Tuesday, April 30, 2002 1:29 PM
> > To: histonet@pathology.swmed.edu
> > Subject: School in Deep Freeze
> >
> > Histo Net:
> >
> > What was the name of that school in Minneapolis, MN that has a Histology
> > program? How can I get in contact with them?
> >
> > I know somebody that my be interested in going this route to become a
> > Histo
> > Tech. The school I went to in Eau Claire, WI has since shut down their
> > program.
> >
> > Thanks in advance.
> >
> > Stephen J. Scholz HT(ASCP)
> > Histology Laboratory
> > OSF St. Anthony Medical Center
> > Rockford Illinois
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 17:17:22 -0500
> From: nina leek
> Subject: Re: DAB and hematoxylin
>
> Amos:
> It's my understanding that the DAB oxidation product is precipitated into
the
> tissue, and probably even chemically bonded to it. So, it doesn't matter
if
> the Ab/Ag complex is dissociated at that point, the stain from DAB will
stay
> put.
> Adrian Leek.
>
>
> Amos Brooks wrote:
>
> > Hi Andi,
> > Just a quick dumb question 'bout that ... Wouldn't the Mayer's
> > hematoxylin disassociate the Ab/Ag complex after the DAB was added too?
I am
> > probably overlooking something and thereby asking a dumb question but
the
> > disassociation of the antibody should occur with or without the DAB when
in
> > the hematoxylin if that was the cause. I don't have any other answer
except
> > to try another hematoxylin and see if it still happens, or to be REALLY
> > careful to counterstain only after the DAB is on (way to state the
obvious
> > right).
> > Amos Brooks
> >
> > ----- Original Message -----
> > From: "Kappeler Andi"
> > To: "Histonet" ; "E Sharon Shields"
> >
> > Sent: Tuesday, April 30, 2002 9:42 AM
> > Subject: Re: DAB and hematoxylin
> >
> > Hi Sharon
> > Hematoxylin has a pH of approximately 2 to 2.5 ... and at this pH all
your
> > antibodies will readily dissociate from their respective antigens,
meaning
> > that all your staining efforts of the last couple of hours are just
gone. No
> > way to save the job, just start over. Sorry to tell you this, but that's
the
> > way it is. Low pH (hematoxylin, acid alcohol, whatever...) - no more
bound
> > antibodies ... and all these low pH reagents will only take seconds to
> > destroy your previous work! Hope this helps.
> >
> > Andi Kappeler
> > Institute of Pathology, University of Bern, Switzerland
> >
> > -----Ursprungliche Nachricht-----
> > Von: E Sharon Shields
> > An:
> > Gesendet: Dienstag, 30. April 2002 15:01
> > Betreff: DAB and hematoxylin
> >
> > > Hello Fellow Histonetters,
> > > I have a question I hope you can answer since it has caused me lots of
> > grief.
> > > Why will IHC slides not signal with DAB if they where first stained
with
> > Mayers Hematoxylin before the DAB was dropped on? On several occasions,
this
> > morning being the latest, Hematoxylin was mistakenly drop on the slides
> > before the DAB. Part of the controls had DAB dropped on them and the
rest
> > where destained with 70% acid alcohol before the DAB was dropped. None
of
> > the control worked. WHY??? These are controls that I know are staining
> > nicely. As I said this is not the first time this has happened. WE need
> > help!!!!!
> > > Thanks,
> > > E.S. Shields
> > > Baptist Hospital of E TN
> > > Knoxville, TN
> > >
> > >
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 17:17:40 -0500
> From: Bryan Hewlett
> Subject: Re: Jenkins decalcifier
>
> I must have been experiencing a 'Senior Moment' when this question was
first
> posted!
> Not too surprising, when you consider that I last used this formulation
> in-----uumm, well---ll, it was along time ago.
> Today, upon suddenly awaking from my late afternoon 'rest', the neurons
seem
> to have kicked in and the following emerged from some dark recess.
> C.E. Jenkins recommended the following alcoholic acid solution for the
> fixation and decalcification of bone.
>
> 73.0 mL... 100% Alcohol, (presumably 74 OP spirit, since I believe he was
> Glaswegian !)
> 10.0 mL .. Distilled water
> 4.0 mL......Concentrated HCl
> 3.0 mL......Glacial acetic acid
> 10.0 mL....Chloroform
>
> He suggested that a human rib would be fixed and decalcified in 48 hours.
> I seem to remember that a longer time was required.
> The reference is; J.Path.Bact., 24: p166, 1921. at least that's what my
> student notes say.
> Yes! I did have to look up the details. But I did remember where they
were.
>
> Bryan
>
> Bryan R. Hewlett
> Technical Specialist
> Anatomical Pathology
> Hamilton Regional Laboratory Medicine Program
> Ontario, Canada
>
>
> - ----- Original Message -----
> From: "Linda Jenkins"
> To:
> Sent: Tuesday, April 30, 2002 10:12 AM
> Subject: Jenkins decalcifier
>
>
> > Sorry, Patsy...I can't lay claim to this formula...sounds interesting
> though!
> > Linda
> >
> > Linda Jenkins, HT
> > Clemson University
> > Dept. of Bioengineering
> > Clemson, SC 29634-0905
> > 864.656.5553
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 19:00:54 -0500
> From: NFitzgi726@aol.com
> Subject: digest
>
>
> digest
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
>
> - --Boundary_(ID_1/8+SrSzlyuUUwSAy3cKXQ)
> Content-type: text/plain; charset=US-ASCII
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_1/8+SrSzlyuUUwSAy3cKXQ)
> Content-type: text/html; charset=US-ASCII
> Content-transfer-encoding: 7BIT
>
> LANG="0">digest
>
> - --Boundary_(ID_1/8+SrSzlyuUUwSAy3cKXQ)--
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 19:15:24 -0500
> From: Melissa Jensen
> Subject: Re: Innoculation of Helicobacter..phase 1
>
> No.At this point it is legal to use tissue that would normally be disposed
> of, as a control...I know there is alot of talk about the Tissue Traffic
> and confidentiality concerning tissue being sent for research.The tissue
we
> are using is for our lab only.Wont be sold or sent to a research lab,nor
is
> there a name attached to it in any way.
> - ----- Original Message -----
> From:
> To:
> Sent: Monday, April 29, 2002 9:30 PM
> Subject: Re: Innoculation of Helicobacter..phase 1
>
>
> > In a message dated 02-04-29 20:46:39 EDT, melzy@indytel.com writes:
> >
> > << For those of us wanting to make our own hp controls read on......I
> talked
> > with our Micro Dept..They purchased hp qc bugs.I talked with our
> Pathologists
> > prior.When the hp came.One pathologist had a fresh autopsy,He gave me a
> > sample of lung.Micro made a broth with the hp.Tissue was incubated >>
> >
> > just curious - do you get specific consent from next of kin to use
autopsy
> > tissues for this purpose?
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 19:19:18 -0500
> From: nina leek
> Subject: Re: Jenkins decalcifier
>
> Bryan:
> The rest seems fair enough, but why the chloroform? Do your notes (or the
> original paper) say anything?
> Also, in the present age when possesion of chloroform without a licence
can
> put
> you in clink for several years, is there an alternative?
> Adrian Leek.
>
>
> Bryan Hewlett wrote:
>
> > I must have been experiencing a 'Senior Moment' when this question was
first
> > posted!
> > Not too surprising, when you consider that I last used this formulation
> > in-----uumm, well---ll, it was along time ago.
> > Today, upon suddenly awaking from my late afternoon 'rest', the neurons
seem
> > to have kicked in and the following emerged from some dark recess.
> > C.E. Jenkins recommended the following alcoholic acid solution for the
> > fixation and decalcification of bone.
> >
> > 73.0 mL... 100% Alcohol, (presumably 74 OP spirit, since I believe he
was
> > Glaswegian !)
> > 10.0 mL .. Distilled water
> > 4.0 mL......Concentrated HCl
> > 3.0 mL......Glacial acetic acid
> > 10.0 mL....Chloroform
> >
> > He suggested that a human rib would be fixed and decalcified in 48
hours.
> > I seem to remember that a longer time was required.
> > The reference is; J.Path.Bact., 24: p166, 1921. at least that's what my
> > student notes say.
> > Yes! I did have to look up the details. But I did remember where they
were.
> >
> > Bryan
> >
> > Bryan R. Hewlett
> > Technical Specialist
> > Anatomical Pathology
> > Hamilton Regional Laboratory Medicine Program
> > Ontario, Canada
> >
> > ----- Original Message -----
> > From: "Linda Jenkins"
> > To:
> > Sent: Tuesday, April 30, 2002 10:12 AM
> > Subject: Jenkins decalcifier
> >
> > > Sorry, Patsy...I can't lay claim to this formula...sounds interesting
> > though!
> > > Linda
> > >
> > > Linda Jenkins, HT
> > > Clemson University
> > > Dept. of Bioengineering
> > > Clemson, SC 29634-0905
> > > 864.656.5553
> > >
> > >
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 20:15:15 -0500
> From: Bryan Hewlett
> Subject: Re: Jenkins decalcifier
>
> Adrian,
>
> My notes say nothing directly, I think I may have the original paper
> somewhere, but don't ask me to find it in a hurry(it would take weeks).
> It would seem to me that, as in Carnoy's fluid, the chloroform is present
> mainly for the removal of fat/lipids. This would speed penetration.
> Most hospital labs in Canada seem to have ready access to chloroform(
maybe
> they have licences?), despite the fact that few use it for processing any
> more.
> All that dreadful phosgene etc. Many still use Carnoy or methacarn for
> fixation of some biopsies.
> I'm unaware of any Techs or Lab managers in clink for possession of it,
> although I am aware of several in the past who kept small private stocks
for
> personal consumption!!
> Alternatives are probably equally obnoxious. Dioxane, THF, petroleum
ether,
> acetone(ouch), diethyl ether(double ouch)come to mind.
>
> Bryan
>
> Bryan R. Hewlett
> Technical Specialist
> Anatomical Pathology
> Hamilton Regional Laboratory Medicine Program
> Ontario, Canada
>
>
>
> - ----- Original Message -----
> From: "nina leek"
> To: "Bryan Hewlett" ;
> Sent: Tuesday, April 30, 2002 8:14 PM
> Subject: Re: Jenkins decalcifier
>
>
> > Bryan:
> > The rest seems fair enough, but why the chloroform? Do your notes (or
the
> > original paper) say anything?
> > Also, in the present age when possesion of chloroform without a licence
> can put
> > you in clink for several years, is there an alternative?
> > Adrian Leek.
> >
> >
> > Bryan Hewlett wrote:
> >
> > > I must have been experiencing a 'Senior Moment' when this question was
> first
> > > posted!
> > > Not too surprising, when you consider that I last used this
formulation
> > > in-----uumm, well---ll, it was along time ago.
> > > Today, upon suddenly awaking from my late afternoon 'rest', the
neurons
> seem
> > > to have kicked in and the following emerged from some dark recess.
> > > C.E. Jenkins recommended the following alcoholic acid solution for the
> > > fixation and decalcification of bone.
> > >
> > > 73.0 mL... 100% Alcohol, (presumably 74 OP spirit, since I believe he
> was
> > > Glaswegian !)
> > > 10.0 mL .. Distilled water
> > > 4.0 mL......Concentrated HCl
> > > 3.0 mL......Glacial acetic acid
> > > 10.0 mL....Chloroform
> > >
> > > He suggested that a human rib would be fixed and decalcified in 48
> hours.
> > > I seem to remember that a longer time was required.
> > > The reference is; J.Path.Bact., 24: p166, 1921. at least that's what
my
> > > student notes say.
> > > Yes! I did have to look up the details. But I did remember where they
> were.
> > >
> > > Bryan
> > >
> > > Bryan R. Hewlett
> > > Technical Specialist
> > > Anatomical Pathology
> > > Hamilton Regional Laboratory Medicine Program
> > > Ontario, Canada
> > >
> > > ----- Original Message -----
> > > From: "Linda Jenkins"
> > > To:
> > > Sent: Tuesday, April 30, 2002 10:12 AM
> > > Subject: Jenkins decalcifier
> > >
> > > > Sorry, Patsy...I can't lay claim to this formula...sounds
interesting
> > > though!
> > > > Linda
> > > >
> > > > Linda Jenkins, HT
> > > > Clemson University
> > > > Dept. of Bioengineering
> > > > Clemson, SC 29634-0905
> > > > 864.656.5553
> > > >
> > > >
> >
> >
> >
> >
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 30 Apr 2002 21:00:56 -0500
> From: RSRICHMOND@aol.com
> Subject: Inoculation of Helicobacter..phase 1
>
> Melissa Jensen (melzy) writes about making cultures of Helicobacter pylori
> for staining controls. (I've translated her post into standard English for
> the convenience of readers abroad.)
>
> I have a lot of reservations about what's been done here. Helicobacter
pylori
> is notoriously difficult to grow in culture media, and I'd be very much
> concerned about overgrowth of other bacteria in an autopsy specimen of
> dubious sterility, particularly because I know that many pathologists are
> unfamiliar with the particular morphology of Helicobacter pylori.
>
> What in the world is "poly floration"?
>
> Why not use known positive tissue as a control?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> >>I talked with our Microbiology department. They purchased Helicobacter
> pylori [standard cultures]. I talked with our pathologists [before doing
> this]. When the H.p. came, [it chanced that] one pathologist had a fresh
> autopsy. He gave me a sample of lung. Microbiology made a broth with the
H.p.
> [and incubated tissue in it]. I asked for a 24 hr sample, and then a 48 hr
> sample. So far I have the 24 hr sample. Autolysis minimal. Four
pathologists
> have looked at the slide. All say Beautiful! However, two pathologists
were
> concerned about the poly floration [sic] I suggested we wait and look at
the
> 48 hr specimen. We are going to plate out the broth to make sure it only
> demonstrates H.p. But it looks like a go! May need to use a more sterile
> tissue. But The H.p. is there! Stay tuned!<<
>
>
> Here are the messages received yesterday!
>
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