Dave,

I just salinate a 0.1 M phosphate buffer, though I usually don't
make as basic a buffer as pH 7.6.

To do that:

Make stock solutions of monobasic and dibasic phosphate

Monobasic 0.2 M
27.8 gm NaH2PO4-H2O to 1L of distilled H2O

Dibasic 0.2 M
28.38 gm Na2HPO4 (anhydrous) to 1L of distilled H2O

Start with a known quantity of stock Dibasic (say 100 ml) and add 
stock Monobasic until the pH is 7.6, using a stirrer and pH meter.  
Adding more dibasic will raise the pH and adding monobasic will
lower it. Once you've reached pH 7.6, dilute the mixture by half 
(double the volume) to obtain a 0.1M buffer.

Saline is just 0.9% NaCl, so add 0.9 gm per 100 ml of buffer. 

Good Luck,

Karen Pawlowski, Ph.D.

DReynolds@SNBLUSA.com wrote:
> 
> Hi folks,
> 
> I searched the archives for a pH 7.6 phosphate buffered saline recipe and
> came up empty.  Does anyone have one to share?  Thanks so much in advance.
> 
> Dave
> 
> David A. Reynolds, HT(ASCP)
> SNBL USA, Ltd.
> 6605 Merrill Creek Parkway
> Everett, WA  98203
> 
> Confidentiality Notice: This email, its contents and attachments are
> confidential and may contain privileged information. It is intended solely
> for the use of addressee(s) only. Any use, copying or disclosure of this
> communication or attachments to any other person is expressly prohibited
> without written permission of SNBL-USA,Ltd. If you receive this message in
> error, please notify the sender at SNBL USA, Ltd. immediately by return
> e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate
> your cooperation.
> 
> -----Original Message-----
> From: Renton, Lousie, Mrs [mailto:177louie@chiron.wits.ac.za]
> Sent: Thursday, May 24, 2001 2:40 AM
> To: histonet@pathology.swmed.edu
> Subject: non-histology thank you
> 
>  Dear all,
> 
> Thank you to those who responded to my posting looking for
> international correspondents. I will forward the information to you all
> individually as soon as I have it.
> 
> best regards
>  Louise