Muscle bx's
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I work with muscle bx's and often run into problems with specimen that arrive
frozen. When they are still frozen and are packed in dry ice. One of the
most frustrating problems that occur with some of the specimens is after I
place the frozen specimen in the cryostat set at-20 the muscle becomes soft
and "squishy." I have placed them directly into the cryostat still frozen as
well as letting them come to room temperature and then snap freezing the
sample. They still seem to get equally soft after they have been in the
cryostat. The muscle acts as if something has caused it to require a lower
temperature to keep it frozen solid. I do not run into that problem with the
muscle bx's that arrive fresh and that I snap freeze.
Can any one suggest good reference books or web sites for established
technique on muscle bx's. I would be very appreciated of any and all hints
from techs who work with muscle bx's.
Thank you so much.
N. Hudson
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>I work with muscle bx's and often run into problems with specimen that arrive
<BR>frozen. When they are still frozen and are packed in dry ice. One of the
<BR>most frustrating problems that occur with some of the specimens is after I
<BR>place the frozen specimen in the cryostat set at-20 the muscle becomes soft
<BR>and "squishy." I have placed them directly into the cryostat still frozen as
<BR>well as letting them come to room temperature and then snap freezing the
<BR>sample. They still seem to get equally soft after they have been in the
<BR>cryostat. The muscle acts as if something has caused it to require a lower
<BR>temperature to keep it frozen solid. I do not run into that problem with the
<BR>muscle bx's that arrive fresh and that I snap freeze.
<BR>
<BR>Can any one suggest good reference books or web sites for established
<BR>technique on muscle bx's. I would be very appreciated of any and all hints
<BR>from techs who work with muscle bx's.
<BR>
<BR>Thank you so much.
<BR>
<BR>N. Hudson
<BR>
<BR>
<BR>
<BR> </FONT></HTML>
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