We have been working on a the Masson's trichrome stain on human uterus.  The
stain looks nice but we are not getting nuclear staining.  We are using
Weigert's Iron Hematoxylin.  It seems to look well when we apply it, and
when we take the tissue out of the stain.  Is it possible that we are losing
staining is subsequent steps due to the acidity, etc?  What can be done
about this?  Increase the mordant concentration?  Change one of the other
steps in the procedure?  It seemed to stain when we did not use Bouin's to
post fix... but then the other dyes did not look well.

thanks,
Katrina