Re: Adenovirus

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From:Phyllis Davie <pdavie@phenopath.com>
To:Cathy Fragiskatos <FRAGISKA@PATHOLOGY.Lan.McGill.CA>, histonet@Pathology.swmed.edu
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Date:Fri, 12 Mar 1999 18:42:49 -0800
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Cathy,
  We are using Chemicon's antibody (cat# MAB805) (clones 20/11 and 2/6).  
We pretreat in 0.01% Pronase at room temperature for 10 minutes.  The 
antibody is run at 1:500 and detected by Vector's Elite ABC technique.  
It seems to be working nicely.


>Dear histonetters,
>
>    Does anyone have any experience with adenovirus testing on 
>paraffin-embedded tissue? What is the most current method available to 
>detect adenovirus infection in tissue?
>
>With many thanks,
>
>Cathy Fragiskatos
>Royal Victoria Hospital
>Montreal, Quebec
>Canada.
>


Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
Seattle, WA
pdavie@phenopath.com
(206)374-9009  phone
(206)374-9009  fax





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