Hi all,

Anybody out there doing VEGF-R1 (Flt) or VEGF-R2 (Flk-1) antibody staining.  We
are presently using antibodies from Santa Cruz on formalin-fixed rat tissue.
Unfortunately, after many tries (varying antigen retrieval, blocking, dilutions,
etc.) we are getting lots of unspecific staining.  I have also been told that
there is no real good control for these specific VEGF's, but lung should stain
positive.

Any thoughts?

Angela Nista
Bayer Corporation
West Haven, Connecticut