We always get lots of staining with VEGF but have not concluded that it is
necessarily non-specific.  Just like Factor 8, VEGF seems to be highly
expressed all over the place in our experience.
Patsy Ruegg

		-----Original Message-----
		From:	angela.nista.b@bayer.com
[mailto:angela.nista.b@bayer.com]
		Sent:	Friday, March 09, 2001 1:28 PM
		To:	histonet@pathology.swmed.edu
		Subject:	VEGF Immuno

		Hi all,

		Anybody out there doing VEGF-R1 (Flt) or VEGF-R2 (Flk-1)
antibody staining.  We
		are presently using antibodies from Santa Cruz on
formalin-fixed rat tissue.
		Unfortunately, after many tries (varying antigen retrieval,
blocking, dilutions,
		etc.) we are getting lots of unspecific staining.  I have
also been told that
		there is no real good control for these specific VEGF's, but
lung should stain
		positive.

		Any thoughts?

		Angela Nista
		Bayer Corporation
		West Haven, Connecticut