Luis,
Yes, you are right. The DAB product will remain. It has a high
substantivity for tissue elements.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Luis Chiriboga <Luis.Chiriboga@med.nyu.edu> 2/March/2001
04:16am >>>
Thanks to all of you who replied to my IHC question. However,  I think I
may have asked the question the wrong way.   I guess what I really
wanted to know was if there is a way to remove the DAB from the
tissue.
I imagine that the antibodies can be eluted off by washing the slides in
a salt solution of high ionic strength,  similar to chromatographic
methods.  I presume this will leave the DAB precipitate in place?
Any thoughts on this???

--
Luis Chiriboga Ph.D, H.T. (ASCP) QIHC
New York University School of Medicine
Department Of Pathology 4W27, Bellevue Hospital
27st & First Avenue
New York, NY 10016