Hi Linda,
we use the following recipe for a 10mM citrate buffer at pH 6,0:

stock solution A (0,1M citric acid): 21,01g C6H8O7 x 2H2O in 1000ml aqua
dest.
stock solution B (0,1M sodium citrate): 29,41g C6H5O7 x 2H2O in 1000ml aqua
dest.

For the buffer take 9ml solution A and 41ml solution B and add 450ml aqua
dest. We use this buffer for microwave antigen retrieval: we put the slides
into a cuvette (after deparaffination) with this buffer and heat at full
power in the microwave until the buffer boils violently. Then we take 5 min
at full power. Make shure you have something to cover the cuvette, otherwise
your concentrations will change drastically. After cooking we let the
sections cool down to approx. 40°C. Then comes the staining.
Good luck and happy staining!

Jochen Schuck

P.S.: In order not to loose your tissue during the cooking process you need
really good adherence of the tissue to the slides....

> Von: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
> Antworten an: histonet@pathology.swmed.edu
> Datum: Mon, 27 Mar 2000 14:42:08 -0600
> An: 'Histonet' <histonet@pathology.swmed.edu>, 'IPOX'
> <ipox-l@pathology.stanford.edu>
> Betreff: citrate buffer recipe
> 
> Our lab would like to make its own citrate buffer for HIER purposes; if
> anyone out there has a "tried and true" recipe for such, we would appreciate
> hearing from you.
> 
> Thanks in advance,
> 
> Linda Sebree
> 
> 
> 
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
> 
> 
> (608)265-6596
> FAX: (608)263-1568
> 
>