Louise,

I've never used it.  My suggestion would be to find a reference lab who
does, perhaps ARUP (Salt Lake City, UT, USA) or  Impath (www.impath.com),
and call or email their technical service departments.

-Teri Johnson
terij@prlnet.com

----- Original Message -----
From: Louise Taylor <louiset@mail.saimr.wits.ac.za>
To: histonet <histonet@pathology.swmed.edu>
Sent: Thursday, March 16, 2000 6:04 AM
Subject: FW: Cathepsin B


>
>
> -
> Hi histonetters,
>
> (I'm resending this message in the hope that someone out there will be
able
> to help. I find it hard to belive that no one has used this antibody.)
>
> Question:
>
>  Has anyone used Cathepsin B (this IS B and not D) on FFPE human tissue?
>
> I am particularly interested in the staining pattern, as the spec sheet
> provided does not give an indication as to whether the staining should be
> membranous or nuclear.  The vendor's technical services are not able to
> assist me either, so its up to you guys!
> Thanks in advance
>
> Louise Taylor
>
>
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa
>
>
>
>
>