Olga,
we use BCL-2 from Dako.  We use the DAKO LSAB2 kit with a 30 minute
incubation in the primary, link, and label.  DAB for 5 minutes.  We HIER for
20 minutes with DAKO"S target Retrieval solution.  We were able to get a
1:25 dilution.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	Olga Podosheva [SMTP:olgpod@hotmail.com]
> Sent:	Wednesday, March 15, 2000 6:27 AM
> To:	histonet@pathology.swmed.edu
> Subject:	
> 
> Hi, I'm Olga. I have a question about Bcl-2 Oncoprotein antibody from 
> DAKO(catalog number is M0887). I'm going to use it on paraffin embedded 
> human tissues. It's new fot our lab, so I'll be happy to receive any kind
> of 
> information. Thank you very much.
> 
>   Olga Podosheva. M.S.
>   Histology Supervisor
>   The NEw York Eye and Ear Infirmary
>   olgpod@hotmail.com
>   opodosheva@NYEE.edu
> ______________________________________________________
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