ihc for beta-gal
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| From: | Patsy.Ruegg@UCHSC.edu |
| To: | histonet@pathology.swmed.edu |
| Reply-To: | |
| Content-Type: | text/plain; charset="iso-8859-1" |
Can you recommend a good method for beta-gal IHC? We have been using the
Sigma GAL-13 antibody which is biotin labeled on tissue briefly fixed in 2%
paraformaldehyde without success. The tissue has been enzyme cytochemically
reacted with a substrate for beta-gal before fixing and processing into
paraffin. Do you suppose this enzyme cytochemical staining is preventing
the IHC labeling of the antigen? Please respond directly to
Philip.Hardy@uchsc.edu on this one.
Patsy Ruegg
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