Tony,

I am sure I can help you on this problem, please come back to me with your
telephone number and I will call you.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: tony.whyte [mailto:tony.whyte@bbsrc.ac.uk]
Sent: 08 March 2000 15:22
To: Reply requested
Subject: Cryosectioning


Hi all,

i'm having trouble sectioning whole frozen mouse embryos.  They were snap
frozen in isopentane in liq N2 and mounted in OCT on cork.  The trouble is
either that the section is compressing or else fragmenting.  Using a new
knife
hasn't helped the problem.  Any ideas?

Tony W.
Cambridge