Hello histonetters,

I joined the list about 2 months ago but haven't posted anything before. I
am an immunologist trying to get acquainted with histotechnology. Presently,
I am dealing with frozen sectioning of mouse muscle specimens and  am not
entirely satisfied with the results: 1) the fibers separate from each other
so that there is large empty spaces between them(probably due to inadequate
freezing); 2) some of the fibers will appear longitudinal while others
rounded (orientation of the specimen?);3)when immunostaining, the edges of
the muscle section as well as its collagen are stained after addition of
substrate.
 
 Would very much appreciate advice on how to improve my technique.

Diana Haddad PhD
Navy Medical Research Institute
Rockville MD 20852