Re: Zinc Formalin (formalin pigment)
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From: | Katie B <bresee98@yahoo.com> |
To: | Histonet Server <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Mon, 21 Jun 1999 08:19:18 -0700 (PDT) |
Content-Type: | text/plain; charset=us-ascii |
The Zinc Formalin I use from Anatech comes in 2 forms. One is buffered
and the other is not and is slightly acidic. Provided tissues are
fixed and processed within a short time, the non-buffered version is ok
for me. But I can see how it may lead to formalin pigment and
corrosion (although I have never had such problems). You may want to
switch to the buffered version which is known as Z-Fix from Anatech.
-Katie
--- "J. A. Kiernan" <jkiernan@julian.uwo.ca> wrote:
> On Sun, 20 Jun 1999 Gervaip@aol.com wrote:
>
> > ... have been using zinc formalin for years now. It is wonderful
> for
> > ... can rust metal lids on specimen jars and formalin pigments are
> > present in the sections.
>
> This is a very interesting observation, because plain acidic
> formaldehyde solutions take quite a while to generate "formalin
> pigment" from haemoglobin. You say that you use the fixative
> in a processor, so presumably you are fixing for a short time
> such as overnight or 24 hours. Is this so? If it is so, then there
> is an implication that formalin pigment generation occurs more
> rapidly in zinc formalin than in simple acidic or non-neutralized
> formalin. This is something everyone should know about. Please
> tell us more!
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University
e-mail: bresee98@yahoo.com
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