My experience with some thing like this, regardless of the substrate, is
that it's an indication of inadequate washing between steps.  Anyone have
any other thoughts?

Bruce W. Brodersen, DVM, PhD   Email:  bbrodersen1@unl.edu
University of Nebraska         Voice:  402 472-1434
Veterinary Diagnostic Center   FAX:    402 472-3094
Lincoln, NE  68583-0907

-----Original Message-----
From: Jamie Erickson [mailto:JErickson@genetics.com]
Sent: Thursday, June 17, 1999 9:34 AM
To: HistoNet@pathology.swmed.edu
Subject: DAB/Nova Red Film




Hi All,
         I've been using the Nova Red from Vector and have notice a red
film over parts of the slides, I've seen this with DAB as well but would
like to correct this any Ideas? It is a Reddish or Brownish film that
sticks to both slides and tissue.

Thanks

Jamie Erickson
Genetics Institute