HI All,
      I know this was discussed sometime ago but could someone tell me the circulation time for BRDU, I believe the dose of BRDU given was 500 ug/kg.  I'm doing a study to evaluate BRDU stained slides of Rat colon and need this info. Also how do people evaluate these slides, is it by number of positive cells/ crypt (how many do you count /slides?) . There seems to be two things to evaluate in these slides migration up the Crypt and proliferation, what's the best way to  evaluate both of these??

If someone could help I'd really appreciate it.

Jamie Erickson