Re: shelf lives
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | "Hendry, Chris I" <HendryC@mar.dfo-mpo.gc.ca> |
Reply-To: | |
Date: | Sun, 13 Jun 1999 02:35:55 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Sat, 12 Jun 1999, Hendry, Chris I wrote:
> I was wondering if anyone had information on the shelf lives of commonly
> used histological chemicals, stock solutions, working solutions, stains,
> etc. I think it would be a good reference for most people if such a list
> existed.
Try Charles J. Cherukian (1997) Manual of the Special Stains
Laboratory. Rochester, NY: Univ. of Rochester Med. Ctr. This is
an excellent book, and can be ordered by way of the URMC web
site (sorry, I don't have their address). In my opinion, nearly
all Chuck's prescribed shelf lives are far too short. Times like
6 months are given for solutions of inorganic compounds that
should keep for ever. Moreover, the lifetimes do not take into
account repeated uses of the same solution: we can reuse Ehrlich's
haematoxylin for 5 years or more, but we usually throw out 1% acetic
acid every day. These practices do not indicate the real stability
of the reagents.
If you need shelf-lives to satisfy your safety inspector, Chuck
Cherukian's book is the one to follow, or at least to say that you
follow. The practical instructions for staining methods are this
book's real treasure: all are set out in the manner of Lee Luna's 1968
AFIP manual, but they are completely up to date.
John Kiernan
London, Canada
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