Lectin stains on blood smears
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| From: | Jane Chambers <jane@cc.usu.edu> |
| To: | "'Histonet'" <histonet@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Wed, 09 Jun 1999 11:39:33 -0600 |
| Content-Type: | text/plain; charset="us-ascii" |
We need to do lectin stains on blood smears. I have done lectin stains on tissues in the past, but have questions about blood smears.
Do I need to do blocking steps (ie: 3% H202/MeOH and protein blocker)?
Do I need to pre-treat with trypsin?
My gut instinct is no to both questions, but would appreciate any insight.
Thank you,
Jane
M. Jane Chambers, MS LAT
USDA/ARS PPRL
1150 East 1400 North
Logan, UT 84341
435-752-2941 (v)
435-753-5681 (f)
jane@cc.usu.edu
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