Remember the cytolyte solution is an alcohol-based fixation. Very little
formalin, so enzyme pre-treatments will tend to chew up the sections.
Try the IPXs leaving out any enzyme unmasking. Some antibodies may not
recognise their antigens (eg p53)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
kold@neo.rr.com
Sent: Saturday, 21 June 2008 1:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] effect on IHC staining with the use of Cytolyt for
cellblocks


Has anybody had any problems with IHC staining on cell block slides that
were processed with Cytolyt solution

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