We had this happen on occassion as well.  We were using biopsy cassettes and 
determined that they (because of the small holes to keep tissue in) were 
allowing air bubbles to be in the cassette.  If you had 1 piece out of 4 
that happened to be in the air bubble, it wouldn't process properly.
Don't know if this is the case in your lab, but we determined that to be our 
problem.
Keep us all posted...another mystery of histology!



Lorraine Cornett, HT (ASCP)
Highlands Pathology
Blue Ridge Division, Kingsport, TN
423 224-5793 fax 423 224-5349





>From: "Joe Nocito" 
>To: "Santana, Diane" 
>,
>Subject: Re: [Histonet] why
>Date: Mon, 12 Jun 2006 18:45:53 -0500
>
>Diane,
>do you the screened cassettes? I ran about 25 GI blocks this morning that 
>sat fixing since Saturday and I had to reprocess 2 of them. This happens 
>every so often and it still puzzles me.
>    Sorry I don't have an answer, but you're not alone with this.
>    There I said it, ( in public too) I don't have all the answers.
>
>Joe
>----- Original Message ----- From: "Santana, Diane" 
>
>To: 
>Sent: Monday, June 12, 2006 11:40 AM
>Subject: [Histonet] why
>
>
>>Hi
>>I am looking for some ideas to help with a problem I am still having with
>>processing my GI bx's. What would cause my tissue to be raw? Not all, not
>>some, only 1 or 2 pieces.
>>Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are
>>beautiful. All my solutions test at the right % and temperatures are fine.
>>Question again, why does 1 piece of tissue come out raw, when the other
>>pieces are fine?
>>thanks in advance,
>>Diane Santana
>>PMA
>>Haverhill, Mass.
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet@lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>--
>>No virus found in this incoming message.
>>Checked by AVG Free Edition.
>>Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006
>>
>>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
FREE pop-up blocking with the new MSN Toolbar – get it now! 
http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet