Re: [Histonet] Species specific antibody marker
Affinity BioReagents, (ABR) has a Mouse MAB (vimentin) which is specific
for Mouse, Human and Rat. (MA3-745). You can use this to distinguish
murine from canine cells, theoretically. I've used a human mitochondria
marker to distinguish human from murine - everything that stains is human
- the ones that don't stain are murine. This should work the same, I
would hope. I haven't found a murine mitochondria marker so far.
Jackie
Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor@abbott.com
"Colin Nixon"
Sent by: histonet-bounces@lists.utsouthwestern.edu
06/17/2005 08:28 AM
Please respond to Colin Nixon
To:
cc:
Subject: [Histonet] Species specific antibody marker
I have received FFPE mouse tissue that has been treated with canine MDCK
cells. It has been established that there are carcinoma cells present in
the mice tissue. What I am trying to establish is whether the tumour has
originated from a canine or murine origin. Does anyone know of any
immunohistochemical markers that would be species specific for this task?
Thanks,
Colin
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