I would be interseted to hear from any lab with experience of rapid
microwave processing of small biopsies for urgent diagnosis. We did have a
machine on demo for a short time and now we are considering the pros and
cons before deciding whether or not to purchase.
Thank you for any help
Graham A McHardy
Pathology dept
Aberdeen Royal infirmary 
01224 553852
> -----Original Message-----
> From:	histonet-request@lists.utsouthwestern.edu
> [SMTP:histonet-request@lists.utsouthwestern.edu]
> Sent:	01 June 2004 18:03
> To:	histonet@lists.utsouthwestern.edu
> Subject:	Histonet Digest, Vol 7, Issue 1 [Scanned By SOPHOS
> Anti-Virus]
> 
> Send Histonet mailing list submissions to
> 	histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
> 	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> 	histonet-request@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
> 	histonet-owner@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>    1. Rabbit anti-FITC-HRP conjugate (Yin Ping Lee)
>    2. VEGF problems in Immunohistochemistry (Miguel Valenzuela)
>    3. apoptosis protocol (anexin,	caspase) for immunohistochemistry
>       (Miguel Valenzuela)
>    4. Older embedding center in ebay. (HCS)
>    5. methyl green counterSTAiNing (Inga Hansson)
>    6. IHC_swine vessels_cross reactivity (Melanie Black)
>    7. RE: IHC_swine vessels_cross reactivity (Flynn, Evelyn)
>    8. RE: caspase 3 and pcna (C.M. van der Loos)
>    9. TRAP staining (Myri37@aol.com)
>   10. RE: Histonet Digest, Vol 6, Issue 9 (Shawn Salesky)
>   11. Re: IHC_swine vessels_cross reactivity (Gayle Callis)
>   12. High resolution with pole piece... (Nejat Y?lmaz)
>   13. Steve Slap (Traczyk7@aol.com)
>   14. Info required  on antibodies (carmen loiselle)
>   15. Oddball formalin question! (Greg Dobbin)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Mon, 31 May 2004 11:57:40 -0700
> From: Yin Ping Lee 
> Subject: [Histonet] Rabbit anti-FITC-HRP conjugate
> To: "'histonet@lists.utsouthwestern.edu'"
> 	
> Message-ID: <6BAF4D075F07D411B30900508B94CBA0C1F564@SERVER20>
> Content-Type: text/plain
> 
> Hi, Histonetter:
>  
> Does anyone have experience with rabbit anti-FITC-HRP conjugate from Dako?
> Does it work not as well and what is the problem exactly?  I am using this
> product for the EBER ISH staining and have occasionally encountered
> mysterious background staining.
>  
> Yin Ping Lee
> Histopathology Lab
> BC Cancer Agency
> Vancouver, BC
>  
>  
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Mon, 31 May 2004 23:09:55 +0200 (CEST)
> From: Miguel Valenzuela 
> Subject: [Histonet] VEGF problems in Immunohistochemistry
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <20040531210955.52279.qmail@web12609.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> Hi all
> 
> I need to find a paper to know what kind of cells are
> stained with VEGF (Vascular endotelial growth
> factor)...
> Yes, I know that endothelial cells are stained but I
> get also stain in adipose cells.
> I want to learn more about this antibody and others
> (tie, angiostatin, VEGF-Receptors 1,2)
> 
> Any help will be useful.
> Thanks very much.
> 
> Miguel Valenzuela.
> Spain.
> 
> __________________________________________________
> Correo Yahoo! - 6 MB, antivirus y antispam ¡gratis! 
>  Regístrate ya - http://correo.yahoo.es 
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Mon, 31 May 2004 23:12:02 +0200 (CEST)
> From: Miguel Valenzuela 
> Subject: [Histonet] apoptosis protocol (anexin,	caspase) for
> 	immunohistochemistry
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <20040531211202.87675.qmail@web12607.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> Hi all
> I would like to contact someone that has used anexin-V
> and/or caspase-3 in paraffin tissues.
> I have a lot of nuclear stain with tunel.
> And I would like to find a protocol.
> 
> Any help will be useful
> Thanks.
> Miguel V-S.
> Spain.
> 
> 
> 
> 		
> ______________________________________________________________________
> Correo Yahoo! - 6MB, más protección contra el spam ¡Gratis!
> http://correo.yahoo.es
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Mon, 31 May 2004 16:56:02 -0700
> From: "HCS" 
> Subject: [Histonet] Older embedding center in ebay.
> To: "Histonet" 
> Message-ID: <000b01c4476a$d55f13e0$6501a8c0@hp>
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> Hi, I have listed a spare embedding center that I no longer need on ebay
> in case anyone would be interested in getting one.   The item number is
> 3818030988.  It is a Reichert-Jung. 
> 
> 
> LeRoy Brown HT(ASCP) HTL
> 
> Histology Consultation Services
> 1-360-966-7300
> www.histocs.com
> 
> Everson, Washington.
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Tue, 1 Jun 2004 07:51:56 +0200
> From: Inga Hansson 
> Subject: [Histonet] methyl green counterSTAiNing
> To: histonet@lists.utsouthwestern.edu
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
> 
> Thank you Sharon C......for the advice!!!!!!
> Maybe I just go and make a new one.......!
> How long do you keep sections in the solution??Is five minutes too
> little??
> 
> Regards
> 
> Inga
> 
> 
> 
> ------------------------------
> 
> Message: 6
> Date: Tue, 1 Jun 2004 10:09:33 +0200
> From: Melanie Black 
> Subject: [Histonet] IHC_swine vessels_cross reactivity
> To: histonet@pathology.swmed.edu
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
> 
> 
> The Abcam mavrophage marker (Ab 8740) and the Dako Mac 387(M0747) 
> claim to cross react with swine. The resin sections would need to de 
> resin the sections first.
> 
> Melanie.
> 
> Dear all,
> 
> I need to identify macrophages and endothelial cells in resin embedded
> pig blood vessels with stent. I couldnt find any specific primary
> antibodies against swine macrophages or endothelial cells and has to
> rely on cross reactivity pirmarily intended in human tissue. Has any one
> tried such cross reactive antibodies in swine tissue or is there
> specific ones against pig which ive missed. Please help
> 
>   J. Raghul, Scientist -C, sri chithra tirunal institute of medical
>   sciences, Trivandrum , India
> 
> -- 
> Cardiovascular Research Unit
> Div. of Cardiothoracic Surgery
> Chris Barnard Building
> University of Cape Town
> Anzio Road
> Observatory
> 7925
> Republic of South Africa
> 
> Tel +27 21 406-6589
> Cel +27 82 469-3352
> Fax +27 21 448-5935
> 
> 
> 
> ------------------------------
> 
> Message: 7
> Date: Tue, 1 Jun 2004 09:56:20 -0400
> From: "Flynn, Evelyn" 
> Subject: RE: [Histonet] IHC_swine vessels_cross reactivity
> To: "Dr. Raghul" ,
> 	histonet@lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=iso-8859-1
> 
> Dear Dr. Raghul,
>      The rabbit polyclonal antibody against von Willebrand factor (Factor
> VIII-related antigen;
> DAKO Cat. # A0082) cross-reacts with most species except rabbit.  I have
> tested it with paraffin
> sections but not with resin, however.
> 
> Sincerely,
> Evelyn Flynn
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dr. Raghul
> Sent: Mon 5/31/2004 12:09 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC_swine vessels_cross reactivity
>  
> Dear all,
> 
> I need to identify macrophages and endothelial cells in resin embedded 
> pig blood vessels with stent. I couldnt find any specific primary 
> antibodies against swine macrophages or endothelial cells and has to 
> rely on cross reactivity pirmarily intended in human tissue. Has any one 
> tried such cross reactive antibodies in swine tissue or is there 
> specific ones against pig which ive missed. Please help
> 
>  J. Raghul, Scientist -C, sri chithra tirunal institute of medical 
>  sciences, Trivandrum , India
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 8
> Date: Tue, 01 Jun 2004 16:16:37 +0200
> From: "C.M. van der Loos" 
> Subject: [Histonet] RE: caspase 3 and pcna
> To: histonet@lists.utsouthwestern.edu
> Cc: pruegg@colobio.com
> Message-ID: <29f0af29f78d.29f78d29f0af@amc.uva.nl>
> Content-Type: text/plain; charset=us-ascii
> 
> Dear Patsy,
> We used a anti-caspase-3 rabbit antibody from Cell Signaling (#9661) on
> FFPE sections in a 1/200 dilution (60 min, RT) after HIER with Tris/EDTA
> pH9.0. This means that the epitope survives both formalin-fixation and
> embedding through alcohols. In your situation you may to fix the cells at
> the chamber slide with 4% PFA (or routinely buffered formalin) for 5 min
> and perform your caspase-3 staining. As it is nuclear staining perhaps you
> need to add 0.1% saponin to all antibody steps and washing buffers to open
> up the cell membranes letting your antibodies go in and out.
> With respect to PCNA (we call it a poorman Ki67!!) you may try to replace
> it by anti-Ki67, rabbit monoclonal SP-6 (Neomarkers). In our hands it
> reacts with human, mouse and rat proliferating nuclei on FFPE's and cryo's
> without any non-specific background staining.
> Hope this helps!
> Chris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center
> Amsterdam - The Netherlands   
> 
> ---- Original Message ----- 
> >From  Patsy Ruegg  
> Date  Thu, 27 May 2004 13:09:18 -0600 
> To  Jackie.O'Connor@abbott.com 
> Cc  histonet@lists.utsouthwestern.edu 
> Subject  [Histonet] caspase 3 and pcna 
> Jackie and all, could you please advise me on doing caspase 3 and pcna on
> cells grown on chamber slides, I mostly need advise with caspase, what
> antibody do you use?
> Thank you all,
> Patsy
> 
> 
> 
> 
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 9
> Date: Tue, 01 Jun 2004 10:11:08 -0400
> From: Myri37@aol.com
> Subject: [Histonet] TRAP staining
> To: histonet@pathology.swmed.edu
> Message-ID: <032BBAD4.24B06E2E.0005167B@aol.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> Hi
> I would like to try SIGMA kit N°85, for staining osteoclats on fresh fixed
> cells (reconstructed tissue, and also paraffin embedded secions. Tissue is
> fixed in 4 % paraformaldehyde, is there another fixative wich works better
> ? 
> Do you think if i must change the way to proceede ?
> 
> Thank you very much
> Myriam baali
> Natural implant
> 
> 
> 
> 
> ------------------------------
> 
> Message: 10
> Date: Tue, 1 Jun 2004 10:54:40 -0400 
> From: Shawn Salesky 
> Subject: [Histonet] RE: Histonet Digest, Vol 6, Issue 9
> To: "'histonet@lists.utsouthwestern.edu'"
> 	, 	Shawn Salesky
> 	
> Message-ID: <9E49B4E3A6200C4B91E5FB99FB1F7EDB571F37@NTMAIL>
> Content-Type: text/plain
> 
> 
> 	Hi All,
> 
> 	I am interested in finding out which CPT code people are using for
> reimbursment of INFORM HPV testing on the Ventana system, from Cytyc
> thin-prep slides.
> 
> 	Any insight would be helpful.
> 
> 
> 
> 
> This e-mail and any attachments is intended only for the person or 
> entity to which it is addressed and may contain confidential and
> privileged information. If you are not the intended recipient,
> please notify the sender immediately by return e-mail, delete this
> e-mail and destroy any copies. Any dissemination or use of this
> information by a person other than the intended recipient is
> unauthorized and may be illegal.
> 
> 
> 
> ------------------------------
> 
> Message: 11
> Date: Tue, 01 Jun 2004 08:59:31 -0600
> From: Gayle Callis 
> Subject: Re: [Histonet] IHC_swine vessels_cross reactivity
> To: "Dr. Raghul" ,
> 	Histonet@lists.utsouthwestern.edu
> Message-ID: <3.0.6.32.20040601085931.00c17958@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> You can try to find the antibodies at SEROTEC, they can tell you what
> cross
> reacts to swine from their cross reactivity chart.  This may be on their
> website.  If the resin is Glycol methacrylate plastic, you will will have
> poor success with immunostaining.  IF the tissue is embedded in
> methylmethacrylate, you can use Neil Hands methods for retrieval, and
> remove the plastic COMPLETELY.  This has been discussed on Histonet
> before,
> you can do a search in Histonet archives.
> 
>  
> 
> At 09:39 AM 5/31/2004 +0530, you wrote:
> >Dear all,
> >
> >I need to identify macrophages and endothelial cells in resin embedded 
> >pig blood vessels with stent. I couldnt find any specific primary 
> >antibodies against swine macrophages or endothelial cells and has to 
> >rely on cross reactivity pirmarily intended in human tissue. Has any one 
> >tried such cross reactive antibodies in swine tissue or is there 
> >specific ones against pig which ive missed. Please help
> >
> > J. Raghul, Scientist -C, sri chithra tirunal institute of medical 
> > sciences, Trivandrum , India
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology 
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 12
> Date: Tue, 1 Jun 2004 18:10:15 +0300
> From: Nejat Y?lmaz 
> Subject: [Histonet] High resolution with pole piece...
> To: "histonet" ,
> 	
> Message-ID: <000801c447ea$8b8b5460$4e01a8c0@mersin.edu.tr>
> Content-Type: text/plain;	charset="windows-1254"
> 
> Hello Again...
> 
> We are wondering resolution of a TEM (i.e. JEM1011) can be increased
> effectively with a part called pole piece? Does it really work?
> Thanks in advance...
> 
> Dr. Necat Yilmaz
> 
> ------------------------------
> 
> Message: 13
> Date: Tue, 1 Jun 2004 11:05:46 EDT
> From: Traczyk7@aol.com
> Subject: [Histonet] Steve Slap
> To: HistoNet@pathology.swmed.edu
> Message-ID: <11a.32fcd3cb.2dedf54a@aol.com>
> Content-Type: text/plain; charset="US-ASCII"
> 
> This message is being posted for Lisa:
> 
> Hello Everyone,
> All of your well wishes and prayers are very much appreciated by Jeremy
> and
> myself. Steven sustained serious injury in the accident. He seems to be
> healing well from most. The most serious is the head trauma, which I
> totally
> believe  he will awaken from (probably asking where the best restaurant
> is). Please keep praying and sending good vibes. I will let you know when
> there is any change. I have been speaking with Dorothy and she offered to
> be
> the contact person. Please contact her for updates. Anything that you
> would
> like to send via email, Dorothy can forward to us.
> 
> Peace and many thanks to you all, Lisa 
> 
> Note from Dorothy:
> Keep those cards and letters coming in.  A reminder, the regular mail
> address 
> is:
> Lisa Smith & Steve Slap
> PO Box 31
> Lake Pleasant, MA 01347
> 
> 
> ------------------------------
> 
> Message: 14
> Date: Tue, 01 Jun 2004 11:46:37 -0400
> From: "carmen loiselle" 
> Subject: [Histonet] Info required  on antibodies
> To: histonet@pathology.swmed.edu
> Message-ID: 
> Content-Type: text/plain; charset=iso-8859-1; format=flowed
> 
> Hello everyone,
> 
> Does anyone have any information regarding the following antibodies;  
> P19(INK4d), Kip1/P27 and fli-1 ???  These antibodies would be used on 
> paraffin sections for immunohistochemistry .  We  also have  Ventana 
> immunostainer (NEXES and Benchmark) .
> 
> 
> Any input would be greatly appreciated . Thank you in advance.
> 
> Carmen
> 
> _________________________________________________________________
> MSN Messenger : discutez en direct avec vos amis !  
> http://messenger.fr.msn.ca/
> 
> 
> 
> 
> ------------------------------
> 
> Message: 15
> Date: Tue, 1 Jun 2004 13:54:59 ADT
> From: "Greg Dobbin" 
> Subject: [Histonet] Oddball formalin question!
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <40BC8AB2.2212.1174A4C@localhost>
> Content-Type: text/plain; charset=US-ASCII
> 
> Hello All,
> Here is this weeks oddball question! I am tring to make latex casts 
> of air sacs in birds (the veterinary people will know that birds have 
> air sacs and pneumatic bones that work in conjuction with the lungs 
> to facilitate O2 uptake [among other functions such as temp. reg.]).
> 
> First of all, we will gently compress the birds body (these birds are 
> found dead and brought in by the public-we are not euthanizing!) to 
> force out excess air. Then, while suspending the bird by the head 
> we will run liquid latex into the trachea. We hope to make casts of 
> the communications between the lungs and the various air sacs 
> and subcutaneous air pockets. Some these diverticuli are quite 
> small so we think we will have to dilute the latex to make it thin 
> enough to pass thru these tiny openings. 
> 
> So since we are diluting the latex anyway, why not dilute it with 
> formalin (10% NBFS) so that we simultaneously begin to fix the 
> surrounding structures? However, diluting the latex means that, by 
> default, the formalin is also being diluted. My question then is this: 
> "What would happen if I made the formalin up partly with latex so 
> that the final concentration of formalin in my latex-formalin mixture, 
> would be 10% and the latex concentration would still be high 
> enough to effectively polymerize?" 
> 
> (I have already tried diluting latex with water and it still polymerized 
> at a dilution of 1:4 following 18 hours at -20 C [checked after 
> thawing of course]). 
> 
> I'm thinking the osmotic pressures would be out of whack and the 
> formalin probably won't penetrate very far from the latex cast 
> anyway, but I thought some you with better knowledge of formalin 
> fixation could offer some insight or suggestions before I start 
> playing around with this. 
> 
> I can't wait see the replies on this one!!
> Cheers!
> Greg  
> 
> 
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Making a living is getting; making a life is giving.
> 
> 
> 
> ------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> End of Histonet Digest, Vol 7, Issue 1
> **************************************

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