The way I've done it is:
1. Rinse tissue in running water for a few hours to get rid of formalin.
2. Pat dry, and freeze as you would any other tissue.
3. Cut sections at anywhere between 4-8 microns.
4. Place slides in absolut Propylene Glycol for 5 min.
5. Transfer slides to Oil Red O for 2-4 hours.
6. Rinse in 85% propylene glycol for 5 min.
7. Counterstain with hematoxylin.
8. Rinse air dry and coverslip with aqueous media.

Hope this helps,

Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Hospital
(210)704-2533
Juan_Gutierrez@srhc.iwhs.org


-----Original Message-----
From: Sean Trombley [mailto:sean.trombley.b@bayer.com]
Sent: Wednesday, June 11, 2003 2:13 PM
To: histonet
Subject: Oil Red O


Hi Everyone!

I was hoping to get a protocol for Oil Red O  on NBF fixed frozen mouse
liver
and intestine.  I would appreciate a freezing protocol as well.   We are
having
a debate in our lab as to the best way to approach this.  Thanks in advance
for
the help!

Sean
Bayer Corp
W. Haven, CT
(203)812-5471