Hi Melissa

HAGA is the name I have seen attached to the modified methenamine silver
method for cerebral amyloid that I mentioned.  I have assumed it was a
contraction of 'Hexamine-(H)-Silver-(AG)-(A)'.  Let me know if you need to
see a method.  As regards cost, it uses a methenamine-silver solution that
is similar to that in a Gomori-Grocott but does not require the initial
chromic acid oxidation step  It requires reduction with formaldehyde after
impregnation but no gold toning.

Bill McMeekin
Senior Chief Biomedical Scientist
Neuropathology
Newcastle General Hospital
UK


-----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: 15 June 2002 23:08
To: histonet@pathology.swmed.edu; McMeekin, Bill
Subject: Re: Alzheimers stain


Bill
   What is HAGA?
----- Original Message -----
From: "McMeekin, Bill" 
To: "'Melissa Jensen'" 
Sent: Friday, June 14, 2002 5:45 AM
Subject: RE: Alzheimers stain


> Melissa
>
> You can stain for plaques and tangles separately.  The modified
methenamine
> silver method (HAGA) is a simple and reliable (for a silver) technique to
> stain senile plaques and diffuse amyloid in AD brain (and if you do
Gomori's
> methenamine silvers for fungi etc. you will have the solutions already).
We
> use the Gallyas method as a silver impregnation method for neurofibrillary
> tangles in AD brain and this gives excellent contrast with no background
> staining (but is a bit more complex and uses slightly more exotic
> solutions).  I would use IHC for beta-Amyloid and PHF-Tau (AT8) by
> preference.
>
> Bill McMeekin
> Senior Chief Biomedical Scientist
> Neuropathology
> Newcastle General Hospital
> UK
> -----Original Message-----
> From: Melissa Jensen [mailto:melzy@indytel.com]
> Sent: 13 June 2002 23:49
> To: histonet@pathology.swmed.edu; Joan Yonchek
> Subject: Re: Alzeheimers stain
>
>
>
> Thanks much Joan! I am wondering now if there is a different silver method
> to use.The reagents for Bielsowskys are numerous.Not sure I want to spend
> that kind of money for a stain we may only use occasionally.Any
> recommendations you have would be appreciated!
>
> ----- Original Message -----
> From: Joan Yonchek 
> To: 'Melissa Jensen' 
> Sent: Thursday, June 13, 2002 4:38 PM
> Subject: RE: Alzeheimers stain
>
> Melissa,
>
> Just sent the fax.  If you try the procedure let me know how it turns out.
>
> Good luck,
>
> Joan
> Regeneration Technologies, Inc
> Alachua, FL  32615
>
> -----Original Message-----
> From: Melissa Jensen [mailto:melzy@indytel.com]
> Sent: Wednesday, June 12, 2002 6:33 PM
> To: Joan Yonchek
> Subject: Re: Alzeheimers stain
>
>
> That would be great! Fax is 319-366-6976
>
> ----- Original Message -----
> From: Joan   Yonchek
> To: 'Melissa Jensen' 
> Sent: Tuesday, June 11, 2002 7:59 AM
> Subject: RE: Alzeheimers stain
>
> Melissa,
>
> Microwave Cookbook for Microscopists
> Art and Science of Visualization
> By  Kok and Boon
>
> Chapter 16, page 250
> Procedure 16.15  Microwave-Stimulated King Silver Staining for
> paraffin-embedded tissues.
> Results:  Neurofibullary tangles and senile plaques are black.
> The procedure states:  The core of the plaque is difficult to stain.  The
> Alzheimer's fibrils will not always be colored.  Lloyd et al. published in
> 1985 a microwave method. We changed their method slightly.
>
> I have never tried this procedure but would be happy to fax you a copy.
>
> Joan
> Regeneration Technologies, Inc
> Alachua, FL  32615
>
>
>
>
>
> At 6:21 PM -0500 9/6/02, Melissa Jensen wrote:
>
> Looking for a microwave stain to demonstrate plaque in the brain.....Can
ya
> help me out?
>
>