Bill
   What is HAGA?
----- Original Message -----
From: "McMeekin, Bill" 
To: "'Melissa Jensen'" 
Sent: Friday, June 14, 2002 5:45 AM
Subject: RE: Alzheimers stain


> Melissa
>
> You can stain for plaques and tangles separately.  The modified
methenamine
> silver method (HAGA) is a simple and reliable (for a silver) technique to
> stain senile plaques and diffuse amyloid in AD brain (and if you do
Gomori's
> methenamine silvers for fungi etc. you will have the solutions already).
We
> use the Gallyas method as a silver impregnation method for neurofibrillary
> tangles in AD brain and this gives excellent contrast with no background
> staining (but is a bit more complex and uses slightly more exotic
> solutions).  I would use IHC for beta-Amyloid and PHF-Tau (AT8) by
> preference.
>
> Bill McMeekin
> Senior Chief Biomedical Scientist
> Neuropathology
> Newcastle General Hospital
> UK
> -----Original Message-----
> From: Melissa Jensen [mailto:melzy@indytel.com]
> Sent: 13 June 2002 23:49
> To: histonet@pathology.swmed.edu; Joan Yonchek
> Subject: Re: Alzeheimers stain
>
>
>
> Thanks much Joan! I am wondering now if there is a different silver method
> to use.The reagents for Bielsowskys are numerous.Not sure I want to spend
> that kind of money for a stain we may only use occasionally.Any
> recommendations you have would be appreciated!
>
> ----- Original Message -----
> From: Joan Yonchek 
> To: 'Melissa Jensen' 
> Sent: Thursday, June 13, 2002 4:38 PM
> Subject: RE: Alzeheimers stain
>
> Melissa,
>
> Just sent the fax.  If you try the procedure let me know how it turns out.
>
> Good luck,
>
> Joan
> Regeneration Technologies, Inc
> Alachua, FL  32615
>
> -----Original Message-----
> From: Melissa Jensen [mailto:melzy@indytel.com]
> Sent: Wednesday, June 12, 2002 6:33 PM
> To: Joan Yonchek
> Subject: Re: Alzeheimers stain
>
>
> That would be great! Fax is 319-366-6976
>
> ----- Original Message -----
> From: Joan   Yonchek
> To: 'Melissa Jensen' 
> Sent: Tuesday, June 11, 2002 7:59 AM
> Subject: RE: Alzeheimers stain
>
> Melissa,
>
> Microwave Cookbook for Microscopists
> Art and Science of Visualization
> By  Kok and Boon
>
> Chapter 16, page 250
> Procedure 16.15  Microwave-Stimulated King Silver Staining for
> paraffin-embedded tissues.
> Results:  Neurofibullary tangles and senile plaques are black.
> The procedure states:  The core of the plaque is difficult to stain.  The
> Alzheimer's fibrils will not always be colored.  Lloyd et al. published in
> 1985 a microwave method. We changed their method slightly.
>
> I have never tried this procedure but would be happy to fax you a copy.
>
> Joan
> Regeneration Technologies, Inc
> Alachua, FL  32615
>
>
>
>
>
> At 6:21 PM -0500 9/6/02, Melissa Jensen wrote:
>
> Looking for a microwave stain to demonstrate plaque in the brain.....Can
ya
> help me out?
>
>