Dear netters: I'm planning a sampling of ruminal papillae (i.e. special
structures endowed with epithelium and lamina propria located in the largest
forestomach of ruminants) in young cattle. The project is aimed at defining
the growth rate of these structures specialised in nutrients absorption, so
it is necessary to estimate the mitotic index. My questions are:

1.What staining would you prefer for seeing DNA and mitosis?
2. Do you consider c-kit to be an adequate marker of the mitotic rate
through IHC in paraffin blocks?

Thank you in advance

Sincerely yours

Agustin Jose Venzano Halliburton
DVM-Pathology Group
INTA, Argentina