Hi  Gratiana,
                     We use a 1:9 ratio H2O2 to methanol.



Jamie Erickson
Associate Scientist
Genetics Institute
1 Burtt Rd.
Andover, MA   01810
work : (978) 247-1348
FAX  : (978) 247-1333

>>> Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au> 06/22 11:20 AM >>>
Hi Histonetters, My name is Gratiana & I'm a PhD student @ The Uni. of Melbourne Australia. My question is what % of methanol that normally used to dilute Hydrogen peroxidase for Hydrogen peroxide quenching in immunohistochemistry.  Thankyou very much for your suggestion.
Regard
Gratiana


BRUCE ABALOZ
HISTOLOGIST		      
DEPARTMENT of ZOOLOGY	           *  ph:    +61 3 93446282
The UNIVERSITY of MELBOURNE        *  fax:   +61 3 93447909 
Parkville, Victoria.3052           *  email: b.abaloz@zoology.unimelb.edu.au                                                                       
AUSTRALIA.
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