Hi Ronnie,

Thanks for your input on this issue. I suspect that although I was assured
that silanised slides were used, in truth,  they were  not. I will, however,
try some of the suggestions (acetone fixation) even though this is not
advocated by all.
best regards
Louise


-----Original Message-----
From: Ronnie Houston [mailto:wee_rory@hotmail.com]
Sent: 16 June 2000 08:02
To: louiset@mail.saimr.wits.ac.za
Subject: muscle immuno


Louise,

We have used Shadon SuperFrost Plus for all our muscle immunohistochemistry
with no problems in section retention. Yes, fat and excessive connective
tissue can be a pathological consequence of some of the muscular
dystrophies, but I haven't found this to affect section adherance. What
thickness are you cutting your sections? Usually 5-6 microns for most of our
work.
I'm assuming you are allowing your sections to completely dry before
commencing the immuno? That could leave to floating problems.

We are now using Fisher Premium Capillary Gap Plus microscope slides (100
micron gap) for all our muscle immunos, as we are now using the Microprobe
system, and getting LM results within 25 minutes, and fluorescence within 1
hour. Great results.

If I can help with anything else on the muscle, neuropath or orthopedic
front don't hesitate to ask; that's my speciality.

Although we are in the States, our lab is staffed by two Scots, one
Durbanite, and one Filipino.

Best wishes

Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
(214) 559 7744
(214) 559 7768 - fax
rhouston@tsrh.org

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