Marilyn,
My colleague and I have a paper in press in Journal of Biochemical and
Molecular Toxicology in which GM-CSF, IL-1 beta, and IL-6 was localized in
formalin fixed paraffin embedded mouse skin tissue. Are you interested in
these cytokines?

Carol
_________
Carol L. Sabourin, Ph.D.
Battelle
Medical Research and Evaluation Facility
505 King Avenue
Columbus, OH 43201-2693
Phone: (614) 424-5024
Fax: (614) 424-5469
sabourinc@battelle.org


> -----Original Message-----
> From:	HistoNet Server [SMTP:histonet@pathology.swmed.edu]
> Sent:	Thursday, June 29, 2000 12:14 PM
> To:	HistoNet Server
> Subject:	Daily Digest
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 28 Jun 2000 12:10:45 -0500
> From: "Tyler, M, Marilyn, Ms" <mtyler@uctgsh1.uct.ac.za>
> Subject: Ihc(mouse tissue)
> 
> Hi All
> A few questions about immunocytochemistry.  Using parafin sections 
> and mouse tissue which make of antibody is recommended for cellular 
> markers as well as cytokine staining?  Any special antigen retrieval 
> methods for T cell markers (specially CD3)?  We have tried citrate 
> buffer pH6.  Which type of trypsin does one normally use?  Any 
> recipies?  Any special tips for background blocking?
> Thanking you
> Marilyn.
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