Re: Cutting Nails

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From:Beckers <msadk@worldnet.att.net>
To:HistoNet Server <histonet@pathology.swmed.edu>
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At the NYS Histology Society's annual conference this spring there was one
presenter who had some good ideas on dealing with nails.  His solution was
to stain free floating nail sections first then mount them on slides and
coverslip them.  100% stay on that way.  Don't have the protocol with me
this evening but I will look it up and send it on when I can.  I recall
many a D-PAS stain for nails or any other stain for that matter that had to
be redone 2 or 3 times before even a shred of nail tissue would remain on
the slide by the end of the stain. If the nail requests were batched 2 or 3
times per week then it would save time and improve turn-around time-right? 
Might as well take the time to learn to do free floating sections than to
reorder, recut,and restain several nail cases a day-huh?? There must be a
simple physical reason nail tissue won't stain adhered to glass.  Anyone
interested in a good research project!?  Just thinking out loud.  When I
find the protocol I will post it-meanwhile-how about some hot pink nail
polish!!!

Sue Becker, HTL
Albany, NY 12205





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