Looking for some help.....I am trying to get CD-23 (Cell Marque, clone 1B12) to stain and am not having very good luck.  I'm using HEIR, 1:50 dilution with 15 minute detection times.  This protocol ( with a few minor adjustments, depending on the antibody)  works well in our lab with other antibodies.  Not so good with this one.  Any suggestions??  Jim B, got any ideas?  Thanks in advance.

Tammy Barnhart, BS, HTL(ASCP)
St. Alexius Medical Center
Bismarck, ND
tbarnhart@primecare.org