Patrick,

To expand a bit on J. Kiernan's comments, it will be very cost effective for 
you to learn to use concentrated antibodies. They are much, much cheaper 
than using predilutes. Using kits for the detection usually makes sense, 
however, due to better consistency between runs. I would suggest contacting 
any of the companies that sell antibodies and ask for a training session 
from their technical rep. They will be happy to help you out with settin up 
your lab.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043




----Original Message Follows----
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
To: "Patrick M. Haley " <pmhales@cybergap.net>
CC: Histonet <HistoNet@Pathology.swmed.edu>
Subject: Re: Immunoperoxidase kits
Date: Wed, 14 Jul 1999 11:27:00 -0400 (EDT)

On Wed, 14 Jul 1999, Patrick M. Haley  wrote:

 > We are researching the idea of offering immunoperoxidase staining. My
 > question is, what companies sell kits that would include primary 
antibody,
 > detection system, chromogen, and control slide in one package? We are on 
a
 > limited budget.

   The idea of a kit is that it includes everything
   _except_ the primary antibody, so that you can use one
   kit to do immunohistochemical tests with lots of
   different primary antibodies. Kits are expensive, and
   if you buy a new one for every different immunohistochemical
   test it won't be long before your limited budget is all in
   the kit company's coffers.

  John A. Kiernan,
  Department of Anatomy & Cell Biology,
  The University of Western Ontario,
  LONDON,  Canada  N6A 5C1







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