Carolyn,

What sort of problems?
We use the Novocastra antibody, find it absolutely reliable and robust,
in a variety of fixatives, with or without demineralization. Following
HIER in citrate buffer pH6.0, we incubate AZF or B5 fixed material for 1
hour at RT in a 1:80 dilution of CD10, formaldehyde fixed sections
overnight in a 1:40 dilution. Detection is with LSAB, AEC as chromogen.
What are you using for control material?  

Regards

Bryan

>----------
>From: 	Luty, Carolyn[SMTP:cluty@is.mgh.mcgill.ca]
>Sent: 	July 8, 1999 2:00 PM
>To: 	Histonet
>Subject: 	CD10
>
>Sorry if this is a repeat question, but we are having problems with CD10
>on paraffin sections. Does anyone have any suggestions? We used antigen
>retrieval with citrate buffer and it still did not work. It is a
>Novacastro antibody.
>
>Carolyn
>Montreal General Hospital.
>
>