Hi Karen
I have worked a lot with frozen mouse tissue, especially intestine from 
foetus. I always use silane coated slides (homemade), and I leave the tissue 
to air dry over night (if it is not fixated, you could fixate it in acetone 
for 5 min. after cutting and then let them air dry). I have the best result 
when fixating the tissue in 4% PFA acouple of hours in the fridge. Hope this 
helps.
Kind regards Andrea


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