I'm kinda paranoid about my sections falling of in IHC (antigen 
retrieval can cause them to fall off, and besides IHC takes so 
long that I don't want to have my sections fall off in my last 
water rinse and have wasted all that time) so I both add 1 tsp of 
gelatin to my water bath and coat my slides with    poly-l-lysine. 
The other person who works in my lab has used gelatin in the water 
bath and then plus slides with good results as well. (I don't use 
plus slides mainly because they are more expensive.)
                                Rebekah Smith
On Thu Jul 22 13:53:25 EDT 2004, "Bonner, Janet" 
 wrote:

> We use Sta-on, a Surgipath product, in our waterbathes when using 
> regular
> slides.  We do not use any adhesive in our waterbath (just plain 
> water) when
> using the "Plus" slides - we find this extremely useful for IHC.
> 
> -----Original Message-----
> From: Dorothy.L.Webb@HealthPartners.Com
> [mailto:Dorothy.L.Webb@HealthPartners.Com]
> Sent: Thursday, July 22, 2004 12:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] water bath adhesives
> 
> 
> 
> I am looking into possibly changing the waterbath adhesive and 
> would like to
> get some input from others as to which products you prefer??  
> Also, aren't
> there some of the adhesives on the market that can interfere with 
> certain
> IHC staining?  Thanks ahead of time for all of the help!!  
> ________________________________________
> 
> This e-mail and any files transmitted with it are confidential 
> and are intended solely for the use of the individual or entity 
> to whom they
> are addressed. If you are not the intended recipient or the 
> individual
> responsible for delivering the e-mail to the intended recipient, 
> please
> be advised that you have received this e-mail in error and that 
> any
> use, dissemination, forwarding, printing, or copying of this 
> e-mail
> is strictly prohibited.
> 
> If you have received this e-mail in error, please immediately 
> notify
> the HealthPartners Support Center by telephone at (952) 967-6600.
> You will be reimbursed for reasonable costs incurred in notifying 
> us.
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> The information contained in this message may be privileged 
> and/or
> confidential and protected from disclosure.  If the reader of 
> this message
> is not the intended recipient or agent responsible for delivering 
> this
> message to the intended recipient, you are hereby notified that 
> any
> dissemination, distribution or copying of this communication is 
> strictly
> prohibited.  If you have received this communication in error, 
> please notify
> the sender immediately by replying to this message and deleting 
> the material
> from any computer. 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet