Yes we use GAL 3 for Thyroid tumours too, along with Cytokeratin 19.
Papillary Ca's are positive with CK19 and mostly negative with Gal 3
while follicular ca's are mostly positive with GAL 3 and mostly negative
with CK19.
See Beesley & McLaren. Histopathology 41. 3. p236 (2002).
 
Lawrence Brett
Royal Infirmary
Edinburgh
Scotland

 
 -----Original Message-----
From: Sawrenko, Christina [mailto:csawrenk@bccancer.bc.ca]
Sent: 21 July 2004 22:07
To: Brett, Lawrence
Subject: RE: [Histonet] Galectin-3



Thanks for the info.  For what application do you use the antibody?  We are
interested in using it for thyroid neoplasms.


Thanks again, 
Chris 

-----Original Message----- 
From: Brett, Lawrence [ mailto:Lawrence.Brett@luht.scot.nhs.uk
 ] 
Sent: Wednesday, July 21, 2004 8:11 AM 
To: 'Sawrenko, Christina' 
Subject: RE: [Histonet] Galectin-3 


We use Novocastra's Galectin 3 but not with CD44v6. Gal 3 works well with 
Tris-EDTA heat antigen retrieval. We use a microwave pressure cooker 
(Biogenex) and incubate for 30 mins in primary diluted at 1:150. 
Hope this is of some value. 

Lawrence Brett 
Immunocytochemistry Lab. 
Royal Infirmary 
Edinburgh 
Scotland 

-----Original Message----- 
From: Sawrenko, Christina [ mailto:csawrenk@bccancer.bc.ca
 ] 
Sent: 16 July 2004 18:21 
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Galectin-3 


Good morning, 
One of our pathologists has asked us to research Galectin-3 for use in 
thyroid neoplasms.  Does anyone have experience with this antibody and do 
you use it in conjuction with CD44v6 (Novocastra)? 
  
Thanks in advance for your help! 
Chris Sawrenko 
Histopathology 
BC Cancer Agency 
Vancouver BC Canada 
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