I have not used Dako but we always use citrate buffer ph6.0 in steamer for
antigen retrieval. Always works!
Annette Featherstone HT/MLT

-----Original Message-----
From: Onnasch, Hanne [mailto:Hanne.Onnasch@agriculture.gov.ie]
Sent: Tuesday, July 20, 2004 04:59
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GFAP Protocol



Hi All,

I'm looking for a protocol for IHC staining for GFAP on paraffin sections.
Does anyone have experience with using the Dako detection kit and an
automated system? 

Regards,
Hanne


Hanne Onnasch 
Research Officer  
Central Veterinary Research Laboratory 
Abbotstown 
Castleknock 
Dublin 15 

phone: +353(0)16072583 
Fax: +353(0)16072604 
hanne.onnasch@agriculture.gov.ie 




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