What kind of microtome are you using?   Soft things need to be cut with a Vibratome.  How thin does it need to be?

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten@myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Wednesday, July 21, 2004 12:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Agarose blocks

I just received tissue in agarose and I tried cutting a couple of them with very little luck.  I have never worked with this before.  Is there a trick to sectioning it?  Should it have been processed differently?  I didn't know what was in the block until I embedded it.

Thank you for any help.

Roberta Horner HT/HTL

 

Animal Diagnostic Lab

Penn State University

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet