I do use freshly prepared fixative and h202 each time.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jorge
Ivan Zapata Valencia
Sent: Saturday, May 15, 2004 12:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fixative and H2O2 for frozen


Hello everybody
a technical question: may I use the same acetone:etanol mix to fix samples
different days, or do I have to prepare it feshly every day?? The same with
the H2O2 PBS solution for endogenous peroxidase blocking. Is it mandatory to
use fresh one?
Thank You very much

jorge ivan zapata

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