BrDU protocol for free floating vibratome sections

From:Radha Nair


Hello,

I am looking for advice about antigen retrieval for BrDU in free floating
vibratome sections (60 microns thick). I have used the citrate
buffer/microwave method with success for paraffin mounted sections and was
wondering if anyone has used this with free floating thick sections...


Thank you very much,

Radha


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				Radha Nair
    		 Department of Zoology, Oxford University

                    		01865271179
			   www.radhagohnair.com
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