Susan,
If Santa Cruz has a monoclonal version of the antibody i would try it for
more specificity.
Patsy

Susan Travers wrote:

> Does anybody have a recommendation for a nNOS (bNOS, NOSI) antibody
> for staining, fixed, frozen-sectioned brain tissue (the nucleus of
> the solitary tract in rat, to be specific). We have been using the
> Santa-Cruz sc-648 rabbit polycolonal, (against the c-terminal end)
> with reasonable success.  However, it is not as sensitive as we would
> like for doing double labeling with immunofluorescence.
>
> thanks for any advice,
>
> susan t
> Susan Travers, Ph.D.
> Associate Professor
> Ohio State University
> College of Dentistry
> Section of Oral Biology
> 305 West 12th Avenue #192
>   P.O. Box 182357
> Columbus, Ohio  43218-2357
> Tel: (614)292-6366
> Fax:614-247-6945
> email: travers.3@osu.edu