Julie and Patsy:

I've witnessed the same DAB fade away.  These were tissues cleared in
xylenes and mounted in Pro Texx or Permount.  Other than working through
the nausea, I don't know what to do.

Anyone else?

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Wed, 10 Jul 2002, rueggp wrote:

> Julie,
> Are you sure the orginal slides were DAB stained and are now faded?  I have no experience with DAB fading, I have seen other chromogens fad such as AEC.  Another
> thought is that with improved enhanced IHC detection systems maybe we are just not used to looking at the lighter staining we used to get.
> Patsy
>
> Julie Clark wrote:
>
> > I have been asked to review some two year-old immuno slides.  Most of the slides are very faded to completely faded.  I have no idea what brand DAB they were using.
> >
> > Has anyone tried to re-stain previously DAB stained immuno slides?  I have to use DAB, I cannot switch chromogens.
> >
> > Is there some way to remove the faded DAB?
> >
> > Thanks,
> >
> > Julie Clark
> > Technical Director
> > Breast Cancer Prevention Center Research Laboratory
> > Room 3021 Lied
> > Univ. of Kansas Med. Center
> > Kansas City, KS
> > Phone: Office: 913-588-3963
> >             Lab:     913-588-3917
> > FAX: 913-588-3821
> > Page: 913-917-6052
>
>
>