Gill's #2 also works very well.  Pam Marcum

> -----Original Message-----
> From: antje.marcantonio@pharma.novartis.com
> [mailto:antje.marcantonio@pharma.novartis.com]
> Sent: Wednesday, July 10, 2002 2:57 AM
> To: r-meyer2@northwestern.edu
> Cc: histonet@pathology.swmed.edu
> Subject: Re: counterstain
>
>
>
> I realized that counterstaining after IHC doesn't depend only on the dye
> but also on the preferences of the person who examines the slides
> afterwards.
> With DAB we like the commercial Mayer's from Dako, because it is giving a
> more light blue counterstain than our selfmade one (used for our HE) which
> results in more dark blue nuclei.
> The time for counterstain is from 10 sec up to 3 min, depending on the
> tissue (FFPE or frozens or zinc fixed) and staining intensity of the IHC.
>
> Antje Marcantonio
> Novartis Pharma AG
> Basle, Switzerland
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