John
The original recipe for Jore's solution (1896) does not contain chloral
hydrate.
Sodium chloride - 1 part
Magnesium sulphate - 2 parts
Sodium sulphate - 2 parts
Formalin - 5 to 10 parts
Distillaed water - 100 parts
The solution is for the preservation of specimens prior to mounting for
museum presentation. When fixation is complete the specimen is washed to
remove all traces of formalin. Colour is restored by treating with 90 then
95% ethanol. Jore recommended the final mounting solution to be equal parts
of glycerin and water.

Jore published a second solution in 1913 which did contain chloral hydrate.
Formalin - 5 parts
Saturated chloral hydrate in distilled water - 5 parts
Artificial Karlsbad salts - 5 parts
Distilled water - 1000 parts
This method supposedly retained color without the need to wash in ethanol.
The specimen was thoroughly washed after fixation and then mounted in  a
sodium acetate/glycerin mixture. This second method did not find much favour
because the addition of chloral hydrate was said to markedly decrease the
formation of methaemoglobin although, in its favor it did decrease
haemolysis.

Kaiserling's method is much preferred if long term preservation of tissues
is required with restoration of color. If color restoration is not required
then neutral buffered formalin (or similar) should be fine to use.
Regards
Roy
mailto:roy.ellis@imvs.sa.gov.au

> -----Original Message-----
> From: J. A. Kiernan [mailto:jkiernan@uwo.ca]
> Sent: Friday, 12 July 2002 02:22 PM
> To: Dave More
> Cc: histonet@pathology.swmed.edu
> Subject: Re: Jores fluid
>
>
> This is a new one for me. What is or was Jores solution?
>
> If it's used for "long-term storage of autopsy tissues"
> it is probably a mixture of water with some cheap
> antiseptic. A pathologist or a histopathology technician
> should be able to tell you what's routinely used for
> human material.
>
> My experience with stored human material is limited to
> brains and spinal cords, which I receive from pathologists
> for research and teaching. These have usually been stored
> for more than a year in neutral formaldehyde. 30% alcohol
> prevents rotting.
>
> I have taken the liberty of forwarding your question to
> the Histonet Listserver. It will be seen by many, and
> some will surely reply.
>
> You can search a veritable goldmine of expertise at
> www.histosearch.com
> This includes some 6+ years of HistoNet and much
> else too. It's up to you, of course, to evaluate
> all that's there.
>
> --
> -------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London,   Canada   N6A 5C1
>    kiernan@uwo.ca
>    http://publish.uwo.ca/~jkiernan/
>
> ------------
>
> Dave More wrote:
> >
> > Hi, John, I was given your name by Cathie Cruckley at LHSC to
> ask about what
> > you use for long-term storage of autopsy tissues.  we have
> always used Jores
>
>
> > solution, but now VWR no longer supplies it and I'm finding that chloral
> > hydrate, which is one of the constituents, is not available in Canada
> > either, so we (so far) can't make it up ourselves.  What do you
> use?  Cheers
> > and thanx in advance, Dave More manager pathology services
> Kingston General
> > Hospital.
>