10%NBF is the recommended and best fixative for FISH.Had some training from
VYSIS on Her2/neu FISH technique.You can also call Vysis technical support
at 1-800-553-7042. Hope this helps

-----Original Message-----
From: Mary Bryhan [mailto:mbryhan@NORTHERNHEALTH.ORG]
Sent: Thursday, June 28, 2001 9:18 AM
To: histonet@pathology.swmed.edu
Subject: Her2/neu FISH


I was told by one of our pathologists that  FISH HER2/neu  isn't affected by
fixation.... opinions??

Mary Bryhan HT (ASCP)
Northern Michigan Hospital
Petoskey, Michigan