We have a Panasonic 600w microwave.
Buffers used are:
Vector pH 6.0
Dako   ph 10
Biogenix pH 10
For all buffers we preheat buffer for 4-5 mins on medium,
on high for 2.5mins to bring Pressure cooker to pressure.
Then 5 mins on medium for retrieval step.
*********************************************************************

thanks, 
that's the one were looking at. Can you tell us how long you antigen 
retrieve and with what buffer?


   __________________________________________ 
   \    Peter Poon                           \     _         ______ | 
    \   http://cafe.berkeley.edu/~peterp      \  /   \___-=0`/|0`/__| 
     \  (510)649-9654                          \_\          / | /    ) 
     /                                         /  `/-==__ _/__|/__ -| 
    /                                         /   *             \ | | 
   /                                         /                   (o) 
   ------------------------------------------ 

On Tue, 1 Aug 2000, Bill Sinai (Anatomical Pathology) wrote:

> Date:          Mon, 31 Jul 2000 14:29:00 -0700 (PDT)
> From:          Peter Poon <peterp@cafe.berkeley.edu>
> Subject:       microwave antigen
> To:            histonet <histonet@pathology.swmed.edu>
> 
> 
> Hi - What brand of microwave pressure cookers do people use for 
> antigen retrieval? We looked on the internet and found pressure 
> cookers ranging from $10 to $60. The $60 dollar one from Nordic Ware 
> looked good, but I was wondering if anyone had had luck with a 
> specific brand. Also, do scientific companies make microwave pressure 
> cookers specifically for antigen retrieval?
> Thanks
> - Peter
> 
> 
>    __________________________________________ 
>   Nordic Ware is the one we have chosen.
> 
> 
> Bill Sinai
> Department Manager
> Tissue Pathology
> ICPMR Westmead Hospital 
> WESTMEAD NSW AUSTRALIA
> Phone 61+2+9845 7774  Fax 61+2+9687 2330
> 


Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330