Hi,
    I have used Proteinase K for 2 minuets and 0.03% H2O2 for 7 minutes.
DAKO has a prediluted antibody which can be used with LSAB2 incubated for 10
minutes, or the concentrate @ (+/-) 1:2000 with LSAB+. The HemePath docs
loved it.
good luck
Amos Brooks

> > Hello all,
> >
> > Was wondering if any kind souls out there could give me some advice on
> > Kappa
> > and Lambda staining on formalin fixed paraffin embedded human tissue.
> > Currently we use tonsil as a control and use HIER by pressure cooking
> > for 2
> > minutes in citrate buffer (pH 6.0).  At the moment we get some
> > background
> > staining.  I have heard that some people use enzyme digestion for
> > these
> > antibodies...any comments??
> >
> > Thanks in advance.
> >
> > Melanie Trivett
> > Peter MacCallum Cancer Institute
> > Melbourne
> > Australia
> >