Re: Looking for Mucin Stains
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From: | Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
"Bourassa, Patricia" wrote:
> Hi, all!
>
> I'm looking for stains for mucins and/or proteoglycans. Someone in my lab
> wants to try to stain cells for surface mucins. Thanks for any help you can
> offer!
>
> Patti Bourassa
> Pfizer, Groton
How about PAS and hematoxylin? Easy to do, very pretty, shows G-I tract
mucins, brush border in kidney tubules, etc. nicely. Formalin, Bouin's or
alcoholic formalin fixation.
Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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