Date sent:      	Fri, 14 Jul 2000 02:46:56 +1000
From:           	Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
Subject:        	Kangaroos & Wallaby's!?
To:             	histoNet@pathology.swmed.edu

> Dear Histonetters.....my name is Gratiana & I am wanting to 
process some VERY tiny/fragile & "precious" specimens of 
Tammar Wallaby Day 12 - 17 vesicles. 

Gratiana, I do quite a lot of work on developing mouse gonads from 
12.5 dpc. mostly frozen sections but some paraffin. Processing 
times are short ( 10-20mins in each) and done in a small conical 
plastic vial, just allow the tissue to sink then carefully decant and 
replace with new solution (a dissecting microscope is useful as 
they are almost invisible unless ISH has been performed). The wax 
is a little tricky but use the same procedure then with the last 
(third) wax allow specimen to sink to the bottom of conical vial, 
cool and solidify, cut off end of vial and invert into base mold, melt, 
remove plastic tip leaving tissue in base mold, block out as usual.
Depending on what you want to do with the specimen it might be 
worth considering staining the tissue prior to processing.
Terry.


Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360